Efficient adenovirus vector‐mediated PPAR gamma gene transfer into mouse embryoid bodies promotes adipocyte differentiation
Efficient adenovirus vector‐mediated PPAR gamma gene transfer into mouse embryoid bodies promotes adipocyte differentiation
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DOI：
10.1002/jgm.1171
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发表时间：
2008-05
期刊：
影响因子：
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通讯作者：
K. Tashiro;K. Kawabata;H. Sakurai;S. Kurachi;F. Sakurai;K. Yamanishi;H. Mizuguchi
中科院分区：
文献类型：
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作者：
K. Tashiro;K. Kawabata;H. Sakurai;S. Kurachi;F. Sakurai;K. Yamanishi;H. Mizuguchi
由于转基因不能整合到宿主基因组中，因此建立一种瞬时基因传递系统，如腺病毒载体，进入胚胎干细胞及其聚集形式--类胚体(EBS)，对于其在再生医学中的应用是至关重要的。在本研究中，我们优化了Ad载体介导的EBS转导，并检测了Ad载体介导的脂肪生成相关基因转导EBS是否能促进脂肪细胞的分化。
Establishment of a transient gene delivery system, such as adenovirus (Ad) vectors, into embryonic stem (ES) cells and their aggregation form, embryoid bodies (EBs), is essential for its application in regenerative medicine because the transgene should not be integrated in the host genome. In this study, we optimized Ad vector‐mediated transduction into EBs, and examined whether Ad vector‐mediated transduction of adipogenesis‐related gene into EBs could promote the adipocyte differentiation.