Inflammatory and immunological parameters of disease activity in rheumatoid arthritis patients treated with minocycline
Inflammatory and immunological parameters of disease activity in rheumatoid arthritis patients treated with minocycline
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DOI：
10.1016/0162-3109(95)00041-0
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Breedveld, FC
中科院分区：
文献类型：
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作者：
Kloppenburg, M;Dijkmans, BAC;Breedveld, FC
本研究的目的是分析米诺环素在类风湿性关节炎中的抗炎作用。研究人员对 65 名 RA 患者的血清样本进行了研究，这些患者完成了为期 26 周的米诺环素（100 毫克，每天两次）与安慰剂的随机双盲试验。在这项试验中，米诺环素治疗组的一些临床参数，特别是急性期反应显着下降。将白蛋白和白细胞介素 6 (IL-6) 的血清水平与 CRP 水平进行比较，以研究急性期反应。此外，还测定了类风湿因子 (RF) 和总免疫球蛋白同种型以及可溶性白细胞介素 2 受体 (sIL2-2R) 的血清水平，以研究该疾病的免疫学参数。通过ELISA测量免疫球蛋白和细胞因子。米诺环素治疗组和安慰剂治疗组的血清白蛋白水平保持稳定，而血清 CRP 水平均下降。仅米诺环素治疗组的血清 IL-6 水平下降，并且这种下降与 CRP 水平的下降呈正相关。米诺环素显着降低血清 IgM-RF、IgA-RF、总 IgM 和总 IgA 水平。此外，米诺环素治疗组中 IgM-RF/总 IgM 的比率下降。在安慰剂治疗组中没有观察到此类变化。 RA患者米诺环素的抗炎作用可能是由于IL-6和类风湿因子合成减少所致。
The objective of this study was to analyze the anti-inflammatory effect of minocycline in rheumatoid arthritis. Serum samples of 65 RA patients who completed a 26-week randomized double-blind trial of minocycline (100 mg twice a day) versus placebo were studied. In this trial some clinical parameters and in particular the acute phase response decreased significantly in the minocycline-treated group. Serum levels of albumin and interleukin-6 (IL-6) were compared with CRP levels in order to study the acute phase response. Furthermore, rheumatoid factor (RF) and total immunoglobulin isotypes as well as serum levels of soluble interleukin-2 receptor (sIL2-2R) were determined in order to study immunological parameters of the disease. Immunoglobulins and cytokines were measured by ELISA. Serum levels of albumin remained stable, whereas serum CRP levels decreased both in the minocycline- and in the placebo-treated group. Serum levels of IL-6 decreased in the minocycline-treated group only and this decrease was positively correlated with the decrease in CRP levels. Minocycline significantly decreased serum IgM-RF, IgA-RF, total IgM and total IgA levels. In addition the ratio of IgM-RF/total IgM decreased in the minocycline-treated group. No such changes were observed in the placebo-treated group. The anti-inflammatory effect of minocycline in RA patients may be due to the reduction in the synthesis of IL-6 and rheumatoid factor.