CONCANAVALIN A CAP FORMATION ON POLYMORPHONUCLEAR LEUKOCYTES OF NORMAL AND BEIGE (CHEDIAK-HIGASHI) MICE
CONCANAVALIN A CAP FORMATION ON POLYMORPHONUCLEAR LEUKOCYTES OF NORMAL AND BEIGE (CHEDIAK-HIGASHI) MICE
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DOI：
10.1038/253471a0
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发表时间：
1975-01-01
期刊：
影响因子：
64.8
通讯作者：
BERLIN, RD
中科院分区：
文献类型：
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作者：
OLIVER, JM;ZURIER, RB;BERLIN, RD
用刀豆球蛋白 A (con A) 处理多种细胞类型会导致凝集素聚集到细胞的一个区域或一极，形成所谓的帽。并非所有细胞都有帽（红细胞和正常培养的成纤维细胞没有）。盖帽的细胞可分为（a）淋巴细胞1和兔渗出多形核白细胞（PMN）（我们的数据），当与低浓度con A在37°C孵育时直接盖帽，以及（b）以SV40转化的成纤维细胞2和淋巴细胞与高（超过5μg ml−1）浓度con A1孵育的细胞为例，仅在与破坏微管的秋水仙碱孵育后才盖帽（公吨）。细胞类型之间的这些差异尚不清楚。
TREATMENT of a variety of cell types with concanavalin A (con A) leads to the aggregation of the lectin into one region or pole of the cell, forming a so-called cap. Not all cells cap (erythrocytes and normal cultured fibroblasts do not). Cells which do cap can be distinguished as (a) lymphocytes1and rabbit exudate polymorphonuclear leukocytes (PMN) (our data) that cap directly when incubated with low concentrations of con A at 37° C, and (b) cells exemplified by SV40-transformed fibroblasts2and lymphocytes incubated with high (more than 5µg ml−1) concentrations of con A1that cap only after incubation with colchicine which disrupts microtubules (MT). These differences between cell types are not understood.