Route to smooth silica-based surfaces decorated with novel self-assembled monolayers (SAMs) containing glycidyl-terminated very long hydrocarbon chains.
Route to smooth silica-based surfaces decorated with novel self-assembled monolayers (SAMs) containing glycidyl-terminated very long hydrocarbon chains.
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通往光滑的二氧化硅基表面，表面装饰有新型自组装单分子层 (SAM)，其中含有缩水甘油基封端的超长烃链。
作者：
D. Dinh;L. Vellutini;B. Bennetau;C. Dejous;D. Rebière;Émilie Pascal;D. Moynet;C. Belin;B. Desbat;C. Labrugère;J. Pillot
合成了一种新型的带有三烷氧基硅基和长碳链（C22）的端甘氨酰有机硅偶联剂。研究了它们在硅基表面接枝形成致密自组装单分子膜的能力。透射红外光谱表明，以三氯乙酸为催化剂（10 M%），得到的薄膜最规则。原子力显微镜（AFM）和光学椭圆偏振仪是一致的，以及有序的单分子膜表现出显着降低的表面粗糙度。落射荧光显微镜显示，这些自组装膜具有更好的表面反应性比单分子层与市售的（3-缩水甘油氧基丙基）三甲氧基硅烷（GPTS）后，接枝的荧光探针（丹酰尸胺）。此外，通过共价结合直接连接的荧光抗体（RAG-TRITC）导致更高的平均荧光强度，表明这些新的自组装膜具有高的潜力，用于生物分子的固定。
Novel glycidyl-terminated organosilicon coupling agents possessing a trialkoxysilyl head group and a very long hydrocarbon chain (C22) were synthesized. Their ability to afford densely packed self-assembled monolayers (SAMs) grafted on silica-based surfaces was investigated. Transmission FT-IR spectra showed that the most regular films were obtained by using trichloracetic acid as the catalyst (10 M%). Atomic force microscopy (AFM) and optical ellipsometry were consistent with well ordered monolayers exhibiting a marked decrease of the surface roughness. Epifluorescence microscopy revealed that these SAMs possessed a better surface reactivity than monolayers obtained with the commercially available (3-glycidoxypropyl) trimethoxysilane (GPTS) upon grafting of a fluorescent probe (dansylcadaverin). Moreover, direct attachment of fluorescent antibodies (RAG-TRITC) through covalent binding led to higher mean fluorescence intensities, showing that these new SAMs possess high potential for the immobilization of biological molecules.