Expression of PvPht1;3, PvACR2 and PvACR3 during arsenic processing in root of Pteris vittata
Expression of PvPht1;3, PvACR2 and PvACR3 during arsenic processing in root of Pteris vittata
复制标题
DOI：
10.1016/j.envexpbot.2020.104312
复制
发表时间：
2021-02
影响因子：
5.7
通讯作者：
Shujun Wei;Y. Kohda;C. Inoue;Mei-fang Chien
中科院分区：
文献类型：
--
作者：
Shujun Wei;Y. Kohda;C. Inoue;Mei-fang Chien
vittais是最著名的砷超蓄积物，但其在分子水平上的机制尚不清楚。在本研究中，砷在磷的吸收和转运。在As加法下，通过时程分析证明了这一点。然后表达3个基因(PvPht1;3，磷酸(P)转运蛋白基因；AsV还原酶基因PvACR2；以AsIII转运基因PvACR3为研究重点，探讨其在甘薯根系As加工中的作用。为害。As加法分析结果表明，p。vittataa对10 ppb AsV也有很高的敏感性，但在6 h内迅速耗尽，而当与P共存时，这种高亲和力受到抑制。对植物中As的分析表明，在没有P的情况下，根部吸收的As在7 d时减少了99%为AsIII， 85%的As以AsIII的形式存在于根状茎中，74%的As积累在叶片中。qRT-PCR结果显示，pvpht1；100 ppb AsV对3的诱导是暂时的，而500 ppb AsV对7 d的诱导是对照的3.7倍。同时，500 ppb AsV对pvacr2的诱导是微弱的（1.15 - 1.45倍），有一定的时滞，且不被P感染。100 ppb AsV对pvacr3的诱导是立即的，强度与AsV浓度呈正相关。有趣的是，pvacr3的表达与根中AsIII的浓度相符。我们的研究结果表明，这三个基因在AsV敏感吸收、组成性AsV减少和随后的AsIII运输中协同作用，导致了p的As超积累。为害。
Pteris vittatais the most well-known arsenic (As) hyperaccumulator, while the corresponding mechanism in molecular level is still not clear. In this study, As uptake and transport inP. vittatawere demonstrated by time-course analyses under As addition. Then expression of 3 genes (PvPht1;3, a phosphate (P) transporter gene;PvACR2, a AsV reductase gene;PvACR3, a AsIII transport gene) was focused on to examine their contributions on As processing in root ofP. vittata. Results of As addition analyses revealed thatP. vittatahad high-sensitivity to even 10 ppb AsV which was quickly depleted within 6 h, while this high affinity was inhibited when coexisting with P. Analyses to As in the plant showed that in the absence of P, 99 % of the As taken up by roots was reduced to AsIII at 7 d. And 85 % of As transported to rhizomes was present as AsIII, 74 % of the As accumulated in fronds was AsIII. Results of qRT-PCR demonstrated that the transcription ofPvPht1;3was temporally induced by 100 ppb AsV without P, while 500 ppb of AsV made this induction kept through the entire period which showed 3.7-fold higher than control at 7 d. Meanwhile,PvACR2was only induced slightly (1.15–1.45-fold) by 500 ppb AsV with a time lag, and this induction wasn’t infected by P.PvACR3was induced by 100 ppb AsV immediately, and the strength was positively related to AsV concentration. Intriguingly, the expression ofPvACR3fitted the AsIII concentration in the root. Our results suggested a collaboration of these three genes in sensitive AsV absorption, constitutive AsV reduction and subsequent AsIII transportation which contributes to As hyperaccumulation byP. vittata.