CRNF, a molluscan neurotrophic factor that interacts with the p75 neurotrophin receptor
CRNF, a molluscan neurotrophic factor that interacts with the p75 neurotrophin receptor
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DOI：
10.1126/science.274.5292.1540
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发表时间：
1996-11-29
期刊：
影响因子：
56.9
通讯作者：
Geraerts, WPM
中科院分区：
文献类型：
--
作者：
Fainzilber, M;Smit, AB;Geraerts, WPM
利用p75神经营养因子受体结合试验，从软体动物滞水蛙中纯化了一种13.1千道尔顿的蛋白质--富含半胱氨酸的神经营养因子（CRNF）。CRNF以纳摩尔亲和力与p75结合，但在序列上与神经营养因子或任何其他已知的基因产物不相似。CRNF信使RNA在成年足上皮下细胞中表达最高;在中枢神经系统中，表达受病变调节。该因子诱发足运动神经元突起生长和调节钙电流。因此，CRNF可能参与运动神经元的靶源性营养支持，并可能代表另一个p75配体家族的原型。
A 13.1-kilodalton protein, cysteine-rich neurotrophic factor (CRNF), was purified from the mollusk Lymnaea stagnalis by use of a binding assay on the p75 neurotrophin receptor. CRNF bound to p75 with nanomolar affinity but was not similar in sequence to neurotrophins or any other known gene product. CRNF messenger RNA expression was highest in adult foot subepithelial cells; in the central nervous system, expression was regulated by lesion. The factor evoked neurite outgrowth and modulated calcium currents in pedal motor neurons. Thus, CRNF may be involved in target-derived trophic support for motor neurons and could represent the prototype of another family of p75 ligands.