IL-10 production in B cells is confined to CD154+ cells in patients with systemic lupus erythematosus
IL-10 production in B cells is confined to CD154+ cells in patients with systemic lupus erythematosus
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DOI：
10.1016/j.jaut.2004.10.001
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发表时间：
2004-12-01
影响因子：
12.8
通讯作者：
Alcocer-Varela, J
中科院分区：
文献类型：
--
作者：
Díaz-Alderete, A;Crispin, JC;Alcocer-Varela, J
SLE的免疫学标志是B细胞过度活跃。CD154 （CD40-L）通常在活化的T细胞中表达，并在T- b相互作用中起重要作用。它在SLE T细胞中的表达增加。此外，在转基因模型中，它在B细胞上的表达导致slea样疾病的发展。IL-10是紊乱SLE免疫系统的关键细胞因子。这项工作的目的是探讨IL-10和CD154在SLE B细胞中的表达之间的关系。我们研究了11名SLE患者和10名健康志愿者。从外周血中分离单个核细胞，在存在或不存在Cowan I株葡萄球菌（CSS）的情况下进行培养。流式细胞术检测细胞表面CD154和胞浆内IL-10的表达。在基础条件下，CD154在患者和对照组中的表达没有差异。B细胞刺激未引起对照B细胞中CD154表达的显著增加。然而，其在SLE患者B细胞中的表达增加了2倍。IL-10的表达仅限于CD154（+）细胞。我们的研究结果表明，IL-10的产生与B细胞中CD154的表达密切相关，并且当sle来源的细胞受到CSS刺激时，IL-10(+) CD154(+) B细胞亚群异常增加。(C) 2004 Elsevier Ltd.版权所有。
The immunological hallmark of SLE is B cell hyperactivity. CD154 (CD40-L) is normally expressed in activated T cells, and plays an important role in T-B interactions. Its expression is increased in SLE T cells. Additionally, its expression on B cells leads to the development of SLE-like disease in a transgenic model. IL-10 is a key cytokine in the disturbed SLE immune system. The aim of this work was to explore the relation between IL-10 and CD154 expression in SLE B cells.We studied 11 SLE patients and 10 healthy volunteers. Mononuclear cells were isolated from peripheral blood and cultured in the presence or absence of Cowan I Strain Staphylococcus (CSS). Surface CD154 and intracytoplasmic IL-10 expression were quantified with flow cytometry.In basal conditions, CD154 expression was not different in patients and controls. B cell stimulation did not cause a significant increase in CD154 expression in control B cells. However, its expression increased 2 times in B cells obtained from SLE patients. IL-10 expression was confined to CD154(+) cells.Our results show that IL-10 production is intimately linked to CD154 expression in B cells, and that the IL-10(+) CD154(+) B cell subset increases abnormally when SLE-derived cells are stimulated with CSS. (C) 2004 Elsevier Ltd. All rights reserved.