Vascular Cell Glycocalyx-Mediated Vascular Remodeling Induced by Hemodynamic Environmental Alteration
Vascular Cell Glycocalyx-Mediated Vascular Remodeling Induced by Hemodynamic Environmental Alteration
复制标题
血流动力学环境改变诱导的血管细胞糖萼介导的血管重塑
DOI：
10.1161/hypertensionaha.117.10678
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发表时间：
2018
期刊：
影响因子：
8.3
通讯作者：
Fan Yubo
中科院分区：
文献类型：
--
作者：
Liu Jiajia;Kang Hongyan;Ma Xuejiao;Sun Anqiang;Luan Huiqin;Deng Xiaoyan;Fan Yubo
血流动力学刺激诱导的血管重塑有助于心血管疾病的病理生理学。在过去的十年中，血管细胞（内皮细胞和平滑肌细胞）糖萼在细胞和分子水平上流动诱导的剪切应力的机械转导中的重要性已得到证明。然而，其在血管重塑中潜在的力传导作用却很少引起人们的关注。在本研究中，使用自制装置将大鼠腹主动脉暴露于无菌、流动或无流动、常压或高压条件下4天。对血管进行组织光度、细胞和分子分析。结果显示，在流量和高压条件下暴露血管后，平滑肌细胞的凋亡率、细胞数量和收缩标记基因平滑肌22的RNA水平均显着增加，而一氧化氮合酶、α-平滑肌肌动蛋白、smoothelin和calponion的表达与流量和常压组相比没有显着差异。此外，血管壁的组织光度分析表明，血流和高压负荷引起的重塑与经典的高血压主动脉表型一致，其特征是血管壁更厚、更坚硬以及主动脉直径增加。然而，通过透明质酸酶处理血管损害糖萼的完整性后，这些现象被完全消除，这提供了血管细胞糖萼在血流动力学刺激诱导的血管重塑中重要的机械转导作用的证据。
Vascular remodeling induced by hemodynamic stimuli contributes to the pathophysiology of cardiovascular diseases. The importance of vascular cells (endothelial cells and smooth muscle cells) glycocalyx in the mechanotransduction of flow-induced shear stress at the cellular and molecular levels has been demonstrated over the past decade. However, its potential mechanotransduction role in vascular remodeling has triggered little attention. In the present study, a home-made apparatus was used to expose the rat abdominal aorta to sterile, flow or no flow, normal-pressure or high-pressure conditions for 4 days. The histomophometric, cellular, and molecular analysis of vessels were performed. The results showed that after exposing the vessels in the flow and high-pressure condition, the apoptotic rate, the cell number, and the RNA level of contractile marker gene smooth muscle 22 of smooth muscle cells were significantly increased, whereas the expression of nitric oxide synthase, &agr;-smooth muscle actin, smoothelin, and calponion showed no significant differences compared with the flow and normal-pressure groups. Moreover, the histomophometric analysis of vascular walls suggested a remodeling induced by flow and high-pressure loading consistent with the classic hypertensive aortic phenotype, which is characterized by a thicker and more rigid vascular wall as well as increased aortic diameter. However, those phenomena were totally abolished after compromising the integrity of glycocalyx by the treatment of vessels with hyaluronidase, which provided evidence of the important mechanotransduction role of the vascular cells glycocalyx in vascular remodeling induced by hemodynamic stimuli.