Effects of ectomycorrhizal fungi on damping‐off and induction of pathogenesis‐related proteins in Pinus tabulaeformis seedlings inoculated with Amanita vaginata
Effects of ectomycorrhizal fungi on damping‐off and induction of pathogenesis‐related proteins in Pinus tabulaeformis seedlings inoculated with Amanita vaginata
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DOI：
10.1111/j.1439-0329.2010.00669.x
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发表时间：
2011-08
期刊：
影响因子：
1.4
通讯作者：
Ru-Qin Zhang;M. Tang;Hui Chen;Z. Tian
中科院分区：
文献类型：
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作者：
Ru-Qin Zhang;M. Tang;Hui Chen;Z. Tian
本文研究了外生菌根真菌（ECMF）对油松幼苗菌根形成的影响、对立枯丝核菌（Rhizoctonia solani）引起的立枯病的保护作用以及在鹅膏菌（Amanita vaginata）和/或R.对Pt幼苗中Solani进行了研究。结果表明：（1）在10种ECMF中，粘球蜱（Gomphidius viscidus）、落叶松乳牛肝菌（Suillus laricinus）、绒毛乳牛肝菌（Suillus tomentosus）、绒山羊乳牛肝菌（A. vaginata和Basidiomycetesno.058）与Pt幼苗形成菌根。菌根侵染水平与保护效果呈正相关（r = 0.9207）。在这5个种中，A. vaginata的侵染水平最高，对立枯病的保护效果最好;粘多糖没有保护作用，即使它具有高的菌根化水平。(ii)几丁质酶、β-1，3-葡聚糖酶和PR-蛋白不受A. vaginata定殖，但当A.阴道菌根幼苗受到R.索拉尼这可能是由于几丁质酶、β-1，3-葡聚糖酶和108、97、78、44、37和14-kDa的PR蛋白都是由A. vaginata和R. solani保护Pt幼苗免受R.茄病感染
Summary The formation of mycorrhiza on Pinus tabulaeformis (Pt) seedlings by ectomycorrhizal fungi (ECMF), the protective effect of the ECMF on damping-off caused by Rhizoctonia solani and the expression of pathogenesis-related proteins (PR-proteins) induced by Amanita vaginata and/or R. solani in Pt seedlings were investigated. The results indicated that: (i) among the 10 ECMF tested, five species (Gomphidius viscidus, Suillus laricinus, Suillus tomentosus, A. vaginata and Basidiomycetes no. 058) formed mycorrhiza with Pt seedlings. There was a positive correlation between mycorrhizal colonization levels and protective effects (r = 0.9207). Among the five species, A. vaginata had the highest colonization level and the best protective effect against damping-off; however, G. viscidus had no protective effect even though it had a high mycorrhization level. (ii) Chitinases, β-1,3-glucanases and PR-proteins in the seedlings were not induced by A. vaginata colonization, but were induced when A. vaginata-mycorrhizal seedlings were attacked by R. solani. It is possible that chitinases, β-1,3-glucanases and 108, 97, 78, 44, 37 and 14-kDa PR-proteins induced both by A. vaginata and R. solani protect Pt seedlings from R. solani infection.