Evidence of early ultrastructural photoreceptor abnormalities in light-induced retinal degeneration using spectral domain optical coherence tomography
Evidence of early ultrastructural photoreceptor abnormalities in light-induced retinal degeneration using spectral domain optical coherence tomography
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DOI：
10.1136/bjophthalmol-2013-304515
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发表时间：
2014-07-01
影响因子：
4.1
通讯作者：
Chavala, Sai H.
中科院分区：
文献类型：
--
作者：
Aziz, Mehak K.;Ni, Aiguo;Chavala, Sai H.
背景：应用光谱域光学相干断层扫描(SD-OCT)技术研究光性视网膜变性小鼠模型视网膜形态和视网膜厚度的时空变化。方法BALB/c小鼠暴露于5000Lux恒定光照3h后，分别于光照后3h、24h、3d、1周和1个月拍摄SD-OCT图像，并与相应时间点的组织学检查结果进行比较。分别于光照后0、1、2 h摄取SD-OCT图像，分析最早时间点视网膜的变化。用Bioptigen软件InVivoVue Divers测量视网膜各层厚度。光照后3h，外核层由低反射变为高反射。24小时时，视网膜外带和核层表现出相似程度的高反射率。外视网膜厚度、玻璃体混浊和视网膜脱离在受伤几天内发生显著变化。伤后1个月可见视网膜变薄。外核层改变先于光感受器节段结构的破坏，最大节段结构改变发生在光照后1~2 h。结论在光损伤模型的早期时间点，SD-OCT显示了组织病理学所不能观察到的视网膜内改变。
Background To study spatiotemporal in vivo changes in retinal morphology and quantify thickness of retinal layers in a mouse model of light-induced retinal degeneration using spectral domain optical coherence tomography (SD-OCT).Methods BALB/c mice were exposed to 5000 lux of constant light for 3 h. SD-OCT images were taken 3 h, 24 h, 3 days, 1 week and 1 month after light exposure and were compared with histology at the same time points. SD-OCT images were also taken at 0, 1 and 2 h after light exposure in order to analyse retinal changes at the earliest time points. The thickness of retinal layers was measured using the Bioptigen software InVivoVue Diver.Results SD-OCT demonstrated progressive outer retinal thinning. 3 h after light exposure, the outer nuclear layer converted from hyporeflective to hyper-reflective. At 24 h, outer retinal bands and nuclear layer demonstrated similar levels of hyper-reflectivity. Significant variations in outer retinal thickness, vitreous opacities and retinal detachments occurred within days of injury. Thinning of the retina was observed at 1 month after injury. It was also determined that outer nuclear layer changes precede photoreceptor segment structure disintegration and the greatest change in segment structure occurs between 1 and 2 h after light exposure.Conclusions Longitudinal SD-OCT reveals intraretinal changes that cannot be observed by histopathology at early time points in the light injury model.