PURIFICATION OF AN N-ETHYLMALEIMIDE-SENSITIVE PROTEIN CATALYZING VESICULAR TRANSPORT
PURIFICATION OF AN N-ETHYLMALEIMIDE-SENSITIVE PROTEIN CATALYZING VESICULAR TRANSPORT
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DOI：
10.1073/pnas.85.21.7852
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
ROTHMAN, JE
中科院分区：
文献类型：
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作者：
BLOCK, MR;GLICK, BS;ROTHMAN, JE
N-乙基马来酰亚胺(NEM)在无细胞系统中抑制高尔基体堆叠连续隔室之间的蛋白质运输。在用NEM使Golig膜失活后，可以通过添加适当制备的胞浆组分来挽救运输。这种互补分析使我们能够提纯NEM敏感因子，我们称之为NSF。NEM敏感因子是一个76 kDa亚基的四聚体，似乎是催化作用的，一个四聚体导致许多运输囊泡的代谢。
N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golig membranes by NEM, transport can be rescued by adding back an appropriately preparaed cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factors is a tetramer of 76-kDa subunits, and appears to act catalytically, one tetramer leading to the metabolism of numerous transport vesicles.