Molecular Combing of λ-DNA using Self-Propelled Water Droplets on Wettability Gradient Surfaces
Molecular Combing of λ-DNA using Self-Propelled Water Droplets on Wettability Gradient Surfaces
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DOI：
10.1021/acsami.6b08607
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发表时间：
2016-09-14
影响因子：
9.5
通讯作者：
Higgins, Daniel A.
中科院分区：
文献类型：
--
作者：
Giri, Dipak;Li, Zi;Higgins, Daniel A.
表面润湿性梯度用于延长和排列双链DNA。通过气相沉积辛基三氯硅烷（C8-硅烷）和氟化辛基三氯硅烷（F-硅烷）前体来制备化合物。水接触角和椭偏膜厚度的测量证实了梯度的形成。将DNA水溶液的液滴放置在每个梯度的疏水端上导致液滴朝向亲水端的自发运动和DNA的沉积。表面吸附的YOYO-1标记的DNA分子的荧光成像显示，它们被拉长，并在沿梯度的所有位置沿着垂直于液滴表面接触线对齐，与表面张力在拉长DNA中发挥的主导作用一致。吸附的DNA的密度被认为是最大的C8-硅烷梯度在其疏水端。DNA密度朝亲水端降低，而伸长的DNA的长度较少依赖于位置。通过化学梯度表面上的自发液滴运动延伸DNA分子在DNA条形码和DNA-蛋白质相互作用的研究中具有可能的应用。
Surface wettability gradients were used to elongate and align double stranded lambda-DNA. Gradients were prepared by vapor phase deposition of octyltrichlorosilane (C8-silane) and fluorinated octyltrichlorosilane (F-silane) precursors. Gradient formation was confirmed by water contact angle and ellipsometric film thickness measurements. Placement of a droplet of aqueous DNA solution on the hydrophobic end of each gradient led to spontaneous motion of the droplet toward the hydrophilic end and deposition of the DNA. Fluorescence imaging of surface-adsorbed YOYO-1 labeled DNA molecules revealed that they are elongated and aligned perpendicular to the droplet-surface contact line at all positions along the gradient, consistent with a dominant role played by surface tension forces in elongating the DNA. The density of adsorbed DNA was found to be greatest on the C8-silane gradient at its hydrophobic end. DNA density decreased toward the hydrophilic end, while the length of the elongated DNA was less dependent on position. The elongation of DNA molecules by spontaneous droplet motion on chemical gradient surfaces has possible applications in DNA barcoding and studies of DNA-protein interactions.