Expression and roles of syndecan-4 in dental epithelial cell differentiation.
Expression and roles of syndecan-4 in dental epithelial cell differentiation.
复制标题
syndecan-4 在牙上皮细胞分化中的表达和作用。
DOI：
10.3892/ijmm.2014.1910
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发表时间：
2014
影响因子：
5.4
通讯作者：
W. Tian
中科院分区：
文献类型：
--
作者：
Zhiling Yan;Guoqing Chen;Yaling Yang;L. Sun;Zongting Jiang;Lian Feng;Mei Yu;W. Guo;W. Tian
Syndecan-4（SDC 4）是一种跨膜硫酸乙酰肝素蛋白聚糖，起着信号转导的作用。它影响许多组织和器官的生长和分化。然而，SDC 4调节牙上皮细胞分化（釉质形成）和牙齿发育的具体机制在很大程度上仍然未知。在本研究中，确定SDC 4调节过程中的牙齿上皮细胞，SDC 4的表达模式进行了检查，在小鼠磨牙和出生后的门牙牙胚在晚钟状发展阶段。设计小干扰RNA（siRNA）用于本研究，并用于下调大鼠牙上皮细胞系HAT-7中SDC 4的表达。结果表明，SDC 4主要存在于口腔上皮、磨牙成釉器的牙上皮细胞和切牙的颈袢中。然而，当内釉上皮细胞产生成釉细胞时，SDC 4表达的丧失是明显的。SDC 4也表达在中间层（SI）细胞中的门牙和牙齿间充质细胞相邻的磨牙（E18）和出生后的门牙的颈环。成纤维细胞生长因子10（FGF 10）促进增殖和轻微降低细胞分化。使用特异性siRNA敲低SDC 4导致细胞增殖减少和釉原蛋白、成釉蛋白、激肽释放酶4和基质金属蛋白酶20表达的高度显著增加，这些分子已知参与釉质的形成。这些作用被FGF 10减弱，FGF 10上调SDC 4表达。这些结果表明，SDC 4参与了牙釉质形成，FGF 10可能通过调控SDC 4的表达来调节牙上皮细胞的行为。
Syndecan-4 (SDC4), a transmembrane heparan sulfate proteoglycan, acts as a signal transducer. It affects the growth and differentiation of a number of tissues and organs. However, the specific mechanisms through which SDC4 regulates the differentiation of dental epithelial cells (amelogenesis) and tooth development remains largely unknown. In the present study, to identify the SDC4-regulated processes in dental epithelial cells, the SDC4 expression pattern was examined in mouse molar and postnatal incisor tooth germs during the late bell stage of development. Small interfering RNA (siRNA) was designed for this study and used to downregulate SDC4 expression in the rat dental epithelial cell line, HAT-7. The results revealed that SDC4 was mainly present in the oral epithelium, the dental epithelial cells of enamel organs in the molars and the cervical loops in the incisors. When the inner enamel epithelial cells gave rise to ameloblasts, however, the loss of SDC4 expression was evident. SDC4 was also expressed in stratum intermedium (SI) cells in the incisors and in dental mesenchymal cells adjacent to the cervical loops in molars (E18) and postnatal incisors. Fibroblast growth factor 10 (FGF10) promoted proliferation and slightly decreased cell differentiation. The knockdown of SDC4 using specific siRNA led to a decrease in cell proliferation and a highly significant increase in amelogenin, ameloblastin, kallikrein 4 and matrix metalloproteinase 20 expression, molecules that are known to participate in the formation of enamel. These effects were attenuated by FGF10, which upregulated SDC4 expression. Taken together, these results suggest that SDC4 participates in amelogenesis, and FGF10 may modulate dental epithelial cell behaviors through the regulation of SDC4 expression.
影响因子：
1.9
作者：
Athanassiou-Papaefthymiou M;Kim D;Harbron L;Papagerakis S;Schnell S;Harada H;Papagerakis P
通讯作者：
Papagerakis P