In vitro stability of endogenous gamma-hydroxybutyrate in postmortem blood.
In vitro stability of endogenous gamma-hydroxybutyrate in postmortem blood.
复制标题
死后血液中内源性γ-羟基丁酸的体外稳定性。
DOI：
10.1520/jfs14443j
复制
发表时间：
1999
影响因子：
1.6
通讯作者：
R. Baselt
中科院分区：
文献类型：
--
作者：
B. G. Stephens;D. E. Coleman;R. Baselt
In vitro Stability of Endogenous Gamma-hydroxybutyrate in References Postmortem Blood 1. Fieler EL, Coleman DE, Baselt RC. g-Hydroxybutyrate concentrations in preand postmortem blood and urine. Clin Chem 1998;44: 692. 2. Anderson DT, Kuwahara T. Endogenous gamma hydroxybutyrate Sir: (GHB) levels in postmortem specimens. Presented at the quarterly meeting of the California Association of Toxicologists, Las Vegas, We and others (1, 2) have previously described the nearly ubiNV, November 4, 1997. quitous presence of apparent ‘‘endogenous’’ GHB (gamma-hy3. Ferrara SD, Tedeschi L, Frison G, Rossi A. Fatality due to gammadroxybutyrate) in postmortem blood at levels that encompass those hydroxybutyric acid (GHB) and heroin intoxication. J Forensic Sci sometimes attributed to GHB overdosage (3–6). We have now 1995;40:501–4. 4. Mozayani A, Brown T, Carter J. The tip of the iceberg in the Harris completed a brief study to determine the importance of storage County Forensic Center. Presented at the annual meeting of the conditions on the stability of GHB in postmortem blood. American Academy of Forensic Sciences, New York, NY, February Blood (and urine, when available) was collected from the infe1997. rior vena cava during the autopsies of 26 adults who died during 5. Fraser DB, Mesmer MZ, Satzger RD. Two fatalities associated with the use of GHB. Presented at the annual meeting of the American August-September, 1997 of circumstances unrelated to GHB Academy of Forensic Sciences, New York, NY, February 20, 1997. usage. The specimens were stored in glass containers with or with6. Woodward W, Todd E. Gamma-hydroxybutyrate. Presented at the out the presence of 10 mg/mL sodium fluoride at either room semiannual meeting of the Southwestern Association of Toxicolo(258C) or refrigerator (48C) temperature. GHB concentrations were gists, San Antonio, TX, April 5, 1997. measured within 2 months by gas chromatography-mass spectro7. Ferrara SD, Tedeschi L, Frison G, et al. Therapeutic gamma-hydroxybutryic acid monitoring in plasma and urine by gas chromametry (7). The results are shown in Table 1. tography-mass spectrometry. J Pharm Biomed Anal 1993;11: It is apparent that room temperature storage had little or no effect 483–7. on ‘‘endogenous’’ GHB concentrations in fluoride-preserved 8. Roth RH. Formation and regional distribution of g-hydroxybutyric blood compared to refrigerated storage. However, the absence of acid in mammalian brain. Biochem Pharm 1970;19:3013-19. fluoride resulted in a 50% higher average GHB value in refrigerated postmortem blood specimens, and the additional factor of room temperature storage of such specimens caused nearly a douBoyd G. Stephens, M.D. bling of the already-elevated GHB content. Chief Medical Examiner Our analysis of nonfluoridated urine for GHB from 17 of the City and County of San Francisco 26 autopsies in this series (14 were undetectable, 3 had 5.1–9.5 San Francisco, CA 94103 mg/L) confirmed our previous experience (1) that postmortem urine does not appear susceptible to the mechanisms that produce Daniel E. Coleman, B.S. such a dramatic rise in GHB levels in postmortem blood. Other Randall C. Baselt, Ph.D. investigators (8) have demonstrated a spontaneous postmortem inChemical Toxicology Institute crease of GHB in brain tissue, and have postulated that GHB is Foster City, CA 94404 formed from GABA or succinic semialdelhyde during conditions of anoxia. We continue to suggest that postmortem urine is a better Commentary on McIntyre IM, King CV, Staikos V, Gall J, Drumspecimen than blood for the investigation of GHB-involved death. mer OH. A fatality involving moclobemide, sertraline and pimozide. J Forensic Sci 1997;42:951–53
