Activated circulating T follicular helper cells and skewing of T follicular helper 2 cells are down-regulated by treatment including an inhaled corticosteroid in patients with allergic asthma
Activated circulating T follicular helper cells and skewing of T follicular helper 2 cells are down-regulated by treatment including an inhaled corticosteroid in patients with allergic asthma
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DOI：
10.1016/j.alit.2019.08.008
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发表时间：
2020-01-01
影响因子：
6.8
通讯作者：
Ichimiya, Shingo
中科院分区：
文献类型：
--
作者：
Miyajima, Satsuki;Shigehara, Katsunori;Ichimiya, Shingo
背景资料：CXCR 5（+）T滤泡辅助（T-FH）细胞主要通过生发中心（GC）促进B细胞产生抗原特异性抗体。T-FH细胞存在于循环中，并且循环（c）T（FH）2细胞（cT（FH）细胞的子集）能够帮助健康个体中的幼稚B细胞产生IgE。相反，产生IL-10的调节性B（布雷格）细胞抑制加速的免疫response.Methods：我们研究了基于特应性哮喘（AA）患者T（H）2应答的cT（FH）细胞和c布雷格细胞的作用。入选32例AA患者和35例健康志愿者（HV）。我们通过流式细胞术检查了cT（FH）细胞，包括其亚群，ICOS和PD-1的表达，以及cBreg细胞及其与临床生物标志物的相关性。结果：与HV组相比，AA组cT（FH）2细胞明显增加，cT（FH）1细胞明显减少。ICOS在cT（FH）及其亚群细胞上的表达水平升高，布雷格细胞上的表达水平显著降低。AA患者血浆CXCL 13水平显著升高，且与cT（FH）2/cBreg比值相关性良好。在10例AA患者吸入皮质类固醇（ICS）治疗前后检查这些细胞。结论：ICS治疗后AA患者T（FH）2和ICOS + cT（FH）2细胞的百分比和数量均下降，且症状和临床生物标志物均得到改善，可作为AA患者T（H）2型气道炎症的生物标志物。Copyright（C）2019，日本变态反应学会制作和主办：Elsevier B. V.
Background: CXCR5(+) T follicular helper (T-FH) cells primarily promote B cells to produce an antigen-specific antibody through germinal centers (GCs). T-FH cells exist in circulation, and circulating(c) T(FH)2 cells, a subset of cT(FH) cells, are able to help naive B cells produce IgE in healthy individuals. Conversely, IL-10-producing regulatory B (Breg) cells inhibit an accelerated immune response.Methods: We investigated the roles of cT(FH) cells and cBreg cells based on a T(H)2 response in patients with atopic asthma (AA). Thirty-two patients with AA and 35 healthy volunteers (HV) were enrolled. We examined cT(FH) cells including their subsets, their expression of ICOS and PD-1, and cBreg cells by flow cytometry and their associations with clinical biomarkers. Plasma levels of CXCL13, which is a counterpart of CXCR5, were also measured using ELISA.Results: In patients with AA, cT(FH)2 cells were increased and cT(FH)1 cells were decreased compared with those in HV. The expression levels of ICOS on cT(FH) and their subset cells were elevated and Breg cells were greatly decreased. The plasma levels of CXCL13 in patients with AA were significantly elevated and correlated well with the cT(FH)2/cBreg ratio. These cells were examined in 10 patients AA before and after inhaled corticosteroid (ICS) treatment. Interestingly, the percentages and numbers of T(FH)2 and ICOS+ cT(FH) cells declined after ICS treatment together with improvements in symptoms and clinical biomarkers.Conclusions: The percentages and numbers of cT(FH)2 and ICOS+ cT(FH) cells might be useful as biomarkers of T(H)2 typed airway inflammation in patients with AA. Copyright (C) 2019, Japanese Society of Allergology. Production and hosting by Elsevier B.V.