Development of an Aqueous Polyethylene Glycol-Based Extraction and Recovery Method for Almond (Prunus armeniaca L.) Protein
Development of an Aqueous Polyethylene Glycol-Based Extraction and Recovery Method for Almond (Prunus armeniaca L.) Protein
复制标题
基于水聚乙二醇的杏仁 (Prunus ameniaca L.) 蛋白提取和回收方法的开发
DOI：
10.1007/s12161-016-0525-3
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发表时间：
2016-12-01
影响因子：
2.9
通讯作者：
Zhang, Zhi-Qi
中科院分区：
文献类型：
--
作者：
Ge, Xian-Li;Shi, Ting;Zhang, Zhi-Qi
以甜杏仁为原料，以聚乙二醇（PEG）水溶液为溶剂，研究了甜杏仁蛋白质的提取及回收方法。考察了不同溶剂（如氢氧化钠、氯化钠、PEG 200、PEG 400和PEG 600水溶液）的萃取率，PEG 200表现出最高的萃取效率。采用响应面法优化了PEG微波辅助萃取的工艺参数。在最佳工艺条件下，PEG 200浓度为25%（w/w），液固比为22 mL g-1，微波功率为120 W，提取温度为45 °C，提取时间为4 min，平均提取率为93.75 ± 3.15%。然后，采用等电点-乙醇协同沉淀法从含PEG的提取液中回收杏仁蛋白。该组合技术综合了等电点沉淀的速度和醇沉的完全性。在3-5 min内，杏仁蛋白的回收率为98.81%.所提出的基于PEG的MAE和协同沉淀方案为杏仁蛋白的提取和回收提供了一种快速有效的方法，并具有用于其他植物蛋白的潜力。
A novel method for protein extraction from sweet almonds with aqueous polyethylene glycol (PEG) as solvent and recovery from the extraction solution was developed. The extraction yields of different solvents, such as sodium hydroxide, sodium chloride, PEG 200, PEG 400, and PEG 600 aqueous solutions, were investigated and PEG 200 showed the highest extraction efficiency. The PEG-based microwave-assisted extraction (MAE) parameters were then optimized using response surface methodology. Under optimum condition, PEG 200 concentration of 25 % (w/w), liquid to solid ratio of 22 mL g−1, microwave power of 120 W, extraction temperature of 45 °C, and extraction time of 4 min, the average extraction yield was 93.75 ± 3.15 %. Subsequently, the almond protein was recovered from the extraction solution containing PEG with an isoelectric point-ethanol synergy precipitation protocol. The combined technique integrated the speed of isoelectric point precipitation with the completeness of alcohol precipitation. The recovery of almond protein was 98.81 % with a time of 3–5 min. The proposed PEG-based MAE and synergy precipitation protocol provide a rapid and effective method for almond protein extraction and recovery and have the potential to be used for other plant proteins.