Somatostatin Inhibition of Basal and Carbachol-Stimulated Gastrin Release in Rat Antral Organ Culture
Somatostatin Inhibition of Basal and Carbachol-Stimulated Gastrin Release in Rat Antral Organ Culture
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生长抑素抑制大鼠胃窦器官培养中基础和卡巴胆碱刺激的胃泌素释放
DOI：
10.1016/0016-5085(81)90495-9
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发表时间：
1981
期刊：
影响因子：
29.4
通讯作者：
J. Mcguigan
中科院分区：
文献类型：
--
作者：
R. Harty;D. G. Maico;J. Mcguigan
在体外条件下的大鼠胃窦器官培养实验中检查了生长抑素对胃泌素释放和总胃泌素免疫反应性的影响。生长抑素抑制基础胃窦胃泌素释放。 10−5M (p < 0.02) 和 10−4M (p < 0.01) 生长抑素显着降低了 6 小时培养物总胃泌素含量（培养基胃泌素加提取的胃窦粘膜胃泌素）。胆碱能药物卡巴胆碱 (10−5M) 刺激的胃泌素释放到培养基中，被生长抑素抑制：在培养 30 分钟和 6 小时，10−8M 生长抑素分别抑制卡巴胆碱刺激的胃泌素释放 66% 和 54%，10−5M 和 10−4M 生长抑素完全消除胃泌素释放。由卡巴胆碱刺激的胃泌素释放速率被所检查的每个剂量的生长抑素（10−8M 至 10−4M）显着抑制。目前的研究表明，生长抑素抑制培养的大鼠胃窦粘膜的胃泌素分泌和胃窦培养胃泌素的总含量，并证明胆碱能介导的胃泌素分泌被生长抑素抑制。
The effects of somatostatin on gastrin release and total gastrin immunoreactivity were examined under in vitro conditions in rat antral organ culture experiments. Basal antral gastrin release was inhibited by somatostatin. Total culture gastrin contents (culture medium gastrin plus extracted antral mucosal gastrin) at 6 h were reduced significantly by 10−5M (p < 0.02) and 10−4M (p < 0.01) somatostatin. Gastrin release into the culture media stimulated by the cholinergic agent, carbachol (10−5M), was suppressed by somatostatin: at 30 min and 6 h of culture 10−8M somatostatin inhibited carbachol-stimulated gastrin release by 66% and 54%, respectively, and 10−5M and 10−4M somatostatin completely abolished gastrin release. The rate of gastrin release stimulated by carbachol was suppressed significantly by each dose of somatostatin examined (10−8M to 10−4M). The present studies indicate that somatostatin inhibits both gastrin secretion by cultured rat antral mucosa and total antral culture gastrin contents, and demonstrate that cholinergically-mediated gastrin secretion is inhibited by somatostatin.