Thermo-responsive chitosan-graft-poly(N-isopropylacrylamide) injectable hydrogel for cultivation of chondrocytes and meniscus cells
Thermo-responsive chitosan-graft-poly(N-isopropylacrylamide) injectable hydrogel for cultivation of chondrocytes and meniscus cells
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DOI：
10.1002/mabi.200600142
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发表时间：
2006-12-08
影响因子：
4.6
通讯作者：
Cheng, Tai-Hong
中科院分区：
文献类型：
--
作者：
Chen, Jyh-Ping;Cheng, Tai-Hong
通过酰胺键将聚N-异丙基丙烯酰胺(PNIPAM)单端羧基接枝到壳聚糖(PNIPAM)上，合成了以壳聚糖为骨架、侧基为基团的温度响应型梳状聚合物。该共聚物具有可逆的温度响应特性，其临界溶解温度(LCST)在30℃左右。扫描电子显微镜观察结果表明，在生理温度下，该共聚物具有多孔的3D水凝胶结构，相互连通的孔径在10~40微米之间。一项初步的体外细胞培养研究表明，这种水凝胶作为一种可注射的细胞载体材料，可以包埋软骨细胞和半月板细胞。这种水凝胶不仅保留了被包裹的细胞的活性和表型，而且还模拟了最初的细胞与细胞的相互作用。
A thermo-responsive comb-like polymer with chitosan as the backbone and pendant poly (N-isopropylacrylamide) (PNIPAM) groups has been synthesized by grafting PNIPAM-COOH with a single carboxy end group onto chitosan through amide bond linkages. The copolymer exhibits reversible temperature-responsive soluble-insoluble characteristics with the lower critical solution temperature (LCST) being at around 30 degrees C. Results from SEM observations confirm a porous 3D hydrogel structure with interconnected pores ranging from 10 to 40 mu m at physiological temperature. A preliminary in vitro cell culture study has demonstrated the usefulness of this hydrogel as an injectable cell-carrier material for entrapping chondrocytes and meniscus cells. The hydrogel not only preserves the viability and phenotypic morphology of the entrapped cells but also simulates the initial cell-cell interactions.