N-acetylcysteine restores the cadmium toxicity of Caenorhabditis elegans
N-acetylcysteine restores the cadmium toxicity of Caenorhabditis elegans
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DOI：
10.1007/s10534-021-00322-z
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发表时间：
2021-06-19
期刊：
影响因子：
3.5
通讯作者：
Matsuoka, Masato
中科院分区：
文献类型：
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作者：
Hirota, Keiko;Matsuoka, Masato
镉是一种众所周知的环境毒物。在细胞水平，暴露于镉通过活性氧（ROS）产生的升高导致细胞毒性效应。尽管镉暴露会导致各种器官功能障碍，但镉在体内毒性作用的潜在机制仍然很大程度上未知。秀丽隐杆线虫（C. habditis elegans）elegans）是一种有用的模式动物，并对环境毒物表现出独特的生物学反应。本研究旨在探讨镉暴露对中华绒螯蟹的毒性机制。使用具有双重功能的N-乙酰半胱氨酸（NAC）研究了线虫，即，作为金属螯合剂和抗氧化剂。NAC没有抑制镉进入线虫的吸收，这表明NAC在这些实验条件下不作为镉的螯合剂。基于这一发现，我们研究了NAC作为抗氧化剂对镉胁迫引起的代表性表型性状的影响-缩短的体长、厌恶行为和缩短的寿命。NAC并没有逆转身体尺寸的减少，但明显恢复了厌恶行为和缩短的寿命。这些数据表明，厌恶行为和缩短寿命介导的氧化应激在C。优雅
Cadmium is a well-known environmental toxicant. At the cellular level, exposure to cadmium results in cytotoxic effects through the elevation of reactive oxygen species (ROS) production. Although cadmium exposure leads to the dysfunction of various organs, the underlying mechanisms of the toxic effects of cadmium in vivo are still largely unknown. Caenorhabditis elegans (C. elegans) is a useful model animal and exhibits unique biological reactions in response to environmental toxicants. In this study, the toxic mechanisms of cadmium exposure in C. elegans were investigated using N-acetylcysteine (NAC), which has dual functions, i.e., as a chelator of metals and as an antioxidant. NAC did not inhibit the uptake of cadmium into nematodes, suggesting that NAC did not function as a chelator of cadmium under these experimental conditions. Based on this finding, we investigated the effect of NAC as an antioxidant on representative phenotypic traits caused by cadmium exposure-reduced body length, aversion behavior, and shortened lifespan. NAC did not reverse the decreased body size but did clearly restore the aversion behavior and the shortened lifespan. These data suggest that aversion behavior and shortened lifespan are mediated by oxidative stress in C. elegans.