Matrix Therapy with RGTA OTR4120 Improves Healing Time and Quality in Hairless Rats with Deep Second-Degree Burns
Matrix Therapy with RGTA OTR4120 Improves Healing Time and Quality in Hairless Rats with Deep Second-Degree Burns
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RGTA OTR4120 基质疗法可改善深二度烧伤无毛大鼠的愈合时间和质量
DOI：
10.1097/prs.0b013e318200a910
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发表时间：
2011
影响因子：
3.6
通讯作者：
D. Barritault
中科院分区：
文献类型：
--
作者：
G. Zakine;Véronique Barbier;S. Garcia;J. Luboinski;D. Papy;J. Chachques;A. Carpentier;D. Barritault
背景：再生剂（RGTA）是可生物降解的聚合物，旨在模拟受损组织细胞外基质中的硫酸乙酰肝素。 RGTA 通过稳定和保护肝素结合生长因子和基质蛋白来改善多种动物模型的组织愈合。 RGTA 恢复正常的基质结构并支持组织再生。在这项研究中，作者评估了 RGTA 对大鼠烧伤模型中表皮修复和真皮重塑的影响。方法：在 156 只无毛大鼠中诱导深二度烧伤，其中一半（n = 78）在烧伤后立即接受局部和肌肉注射 RGTA，然后每周肌肉注射 RGTA，持续 1 个月。对照组（n = 78）根据相同的方案接受生理盐水。从第一周的每一天开始以及第14、28、60、120、240和365天处死大鼠。通过摄影、组织学和免疫组织化学评估烧伤情况。结果：凝固性坏死累及整个表皮及浅表附件。与对照组相比，根据细胞层数和抗细胞角蛋白 14 染色在第 3 天和第 7 天之间评估，RGTA 组的表皮修复速度更快；根据真皮中的继发性血管病变评估，瘀滞区域更小。第7天，两组均完成了上皮再生。第 14 天和第 28 天，RGTA 组重塑的真皮区域较小。结论：RGTA 加速表皮修复并保护真皮免受热的二次影响（通过瘀滞区大小和真皮重塑程度来量化）。
Background: ReGeneraTing Agents (RGTAs) are biodegradable polymers engineered to mimic heparan-sulfate in the extracellular matrix of damaged tissue. RGTAs improve tissue healing in several animal models by stabilizing and protecting heparin-binding growth factors and matrix proteins. RGTA restores the normal matrix architecture and supports tissue regeneration. In this study, the authors evaluated the effects of RGTA on epidermal repair and dermal remodeling in a rat burn model. Methods: Deep second-degree burns were induced in 156 hairless rats, of which half (n = 78) received topical and intramuscular RGTA immediately after the burn followed by intramuscular RGTA weekly for 1 month. The controls (n = 78) received saline according to the same protocol. Rats were killed starting on each day of the first week and on days 14, 28, 60, 120, 240, and 365. The burns were evaluated by photography, histology, and immunohistochemistry. Results: Coagulation necrosis involved the entire epidermis and superficial adnexa. Compared with the controls, speed of epidermal repair, as assessed between days 3 and 7 based on cell-layer number and anticytokeratin-14 staining, was faster in the RGTA group; and the zone of stasis, as assessed based on secondary vascular lesions in the dermis, was smaller. On day 7, reepithelialization was complete in both groups. On days 14 and 28, the remodeled dermal zone was smaller in the RGTA group. Conclusion: RGTA accelerated epidermal repair and protected the dermis from secondary effects of heat as quantified by zone-of-stasis size and extent of dermal remodeling.