A radiobinding assay for human H-Y antigen using monoclonal antibodies.
A radiobinding assay for human H-Y antigen using monoclonal antibodies.
复制标题
使用单克隆抗体对人 H-Y 抗原进行放射性结合测定。
DOI：
10.1097/00007890-198401000-00006
复制
发表时间：
1984
期刊：
影响因子：
6.2
通讯作者：
Koo,GC
中科院分区：
文献类型：
--
作者：
Nagamine,C;Reidy,J;Koo,GC
Radiobinding assays using monoclonal HY antibodies were performed on human peripheral blood leukocytes, concanavalin-A-(conA) 3 stimulated blast cells, and fibroblast primary cell cultures. For peripheral blood leukocytes, male-specific binding was demonstrated only when the cells were enriched for T cells and the HY antisera were partially purified for the IgG or IgM fractions. Similar results were obtained with con-Astimulated blast cells. Fibroblast cells could be tested with unpurified HY antisera. With our monoclonal antibodies, best results were obtained using con A blast cells and fibroblasts.
