Sequence divergence and expression of a transformer gene in the branchiopod crustacean, Daphnia magna
Sequence divergence and expression of a transformer gene in the branchiopod crustacean, Daphnia magna
复制标题
DOI：
10.1016/j.ygeno.2009.12.005
复制
发表时间：
2010-03-01
期刊：
影响因子：
4.4
通讯作者：
Iguchi, Taisen
中科院分区：
文献类型：
--
作者：
Kato, Yasuhiko;Kobayashi, Kaoru;Iguchi, Taisen
环境的衰退触发了大型蚤的生殖策略从无性生殖到有性生殖的转变，然而，这种环境性别决定的分子基础在很大程度上仍然未知。在与鳃足类甲壳动物最密切相关的昆虫中，果蝇Transformer基因的直系同源物如双翅目Transformer（tra）、蜜蜂雌性化者（fern）和互补性别决定者（csd）起性别决定因子的作用。因此，我们克隆了一个D。magna transformer Transformer基因（dmagtra），并分析其表达。预测的氨基酸序列由380个氨基酸组成，包括富含精氨酸丝氨酸的区域，这是Tra的昆虫直向同源物的特征。残基180至205与已知的Tra直系同源物高度保守。DmagTra的结构域组织不同于已知的Tra直系同源物;此外，DmagTra在表达或剪接模式中不显示任何可检测的性二态差异。我们从这些结果推断，dmagtra可能不负责性别决定的D。magna. (C)2010年爱思唯尔公司All rights reserved.
Environmental decline triggers a switch in reproductive strategy of Daphnia magna from asexual to sexual reproduction; however, the molecular basis of such environmental sex determination remains largely unknown. In insects most closely related to branchiopod crustaceans, orthologs of the Drosophila transformer gene such as dipteran transformer (tra), honeybee feminizer (fern) and complementary sex determiner (csd) function as sex determining factors. Therefore, we cloned a D. magna transformer gene (dmagtra) and analyzed its expression. The predicted amino acid sequence has 380 amino acids including an arginine serine-rich region, which is characteristic of insect orthologs of Tra. Residues 180 to 205 are highly conserved with known Tra orthologs. The domain organization of DmagTra is distinct from known Tra orthologs; moreover, dmagtra does not display any detectable sexual dimorphic differences in expression or splicing patterns. We infer from these results that dmagtra may not be responsible for sex determination in D. magna. (C) 2010 Elsevier Inc. All rights reserved.