Ultrasound ionization of biomolecules

Ultrasound ionization of biomolecules
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DOI:
10.1002/rcm.4677
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发表时间:
2010-09-01
影响因子:
2
通讯作者:
Chen, Chung-Hsuan
Chen, Chung-Hsuan
中科院分区:
化学3区
文献类型:
--
作者:
Wu, Chen-I;Wang, Yi-Sheng;Chen, Chung-Hsuan

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迄今为止,生物分子的质谱分析主要是通过基质辅助激光解吸/电离(MALDI)或电喷雾电离(ESI)进行的。在这项工作中,由一个简单的压电装置产生的超声波被证明是生物分子软电离的一种替代方法。蛋白质、糖类和脂肪酸的前体离子几乎没有碎裂。空化被认为是生物分子电离的主要机制。版权所有John Wiley & Sons, Ltd。
To date, mass spectrometric analysis of biomolecules has been primarily performed with either matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization (ESI). In this work, ultrasound produced by a simple piezoelectric device is shown as an alternative method for soft ionization of biomolecules. Precursor ions of proteins, saccharides and fatty acids showed little fragmentation. Cavitation is considered as a primary mechanism for the ionization of biomolecules. Copyright (C) 2010 John Wiley & Sons, Ltd.