Neomycin and pentagalloyl glucose enhanced cross-linking for elastin and glycosaminoglycans preservation in bioprosthetic heart valves.
Neomycin and pentagalloyl glucose enhanced cross-linking for elastin and glycosaminoglycans preservation in bioprosthetic heart valves.
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DOI：
10.1177/0885328213479047
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发表时间：
2014-01
影响因子：
2.9
通讯作者：
Vyavahare NR
中科院分区：
文献类型：
--
作者：
Tripi DR;Vyavahare NR
戊二醛交联生物人工心脏瓣膜在植入12-15年内由于耐久性有限而失效。戊二醛不能充分稳定细胞外基质成分，如糖胺聚糖和弹性蛋白，这些成分的丢失可能是瓣膜植入后变性的主要原因。我们之前已经表明，新霉素基交联可以稳定组织中的糖胺聚糖，但不能稳定弹性蛋白成分。在这里，我们报道了一种新的治疗方法，将新霉素和五棓酰葡萄糖（PGG）掺入戊二醛交联的新霉素-PGG-戊二醛（NPG）中，以稳定猪主动脉瓣中的糖胺聚糖和弹性蛋白。体外研究表明，与戊二醛对照相比，在交联和10个月的储存后，NPG组细胞外基质抗酶降解的稳定性显著增加。拉伸性能显示，与戊二醛相比，NPG组在径向和周向的下弹性模量均有所增加，这可能是由于弹性蛋白的稳定性增加，而上弹性模量和延伸性没有变化。大鼠皮下植入三周后，npg处理组织的细胞外基质稳定性进一步增强。与戊二醛对照组相比，NPG组也显示钙化减少。我们得出结论，NPG交联将是戊二醛交联生物人工心脏瓣膜的一个很好的替代品，以提高其耐久性。
Glutaraldehyde cross-linked bioprosthetic heart valves fail within 12–15 years of implantation due to limited durability. Glutaraldehyde does not adequately stabilize extracellular matrix components such as glycosaminoglycans and elastin, and loss of these components could be a major cause of degeneration of valve after implantation. We have shown earlier that neomycin-based cross-linking stabilizes glycosaminoglycans in the tissue but fails to stabilize elastin component. Here, we report a new treatment where neomycin and pentagalloyl glucose (PGG) were incorporated into glutaraldehyde cross-linking neomycin-PGG-Glutaraldehyde (NPG) to stabilize both glycosaminoglycans and elastin in porcine aortic valves. In vitro studies demonstrated a marked increase in extracellular matrix stability against enzymatic degradation after cross-linking and 10 month storage in NPG group when compared to glutaraldehyde controls. Tensile properties showed increased lower elastic modulus in both radial and circumferential directions in NPG group as compared to glutaraldehyde, probably due to increased elastin stabilization with no changes in upper elastic modulus and extensibility. The enhanced extracellular matrix stability was further maintained in NPG-treated tissues after rat subdermal implantation for three weeks. NPG group also showed reduced calcification when compared to glutaraldehyde controls. We conclude that NPG cross-linking would be an excellent alternative to glutaraldehyde cross-linking of bioprosthetic heart valves to improve its durability.