Placenta growth factor is over-expressed and has prognostic value in human breast cancer
Placenta growth factor is over-expressed and has prognostic value in human breast cancer
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DOI：
10.1016/j.ejca.2005.07.022
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发表时间：
2005-12-01
影响因子：
8.4
通讯作者：
Jiang, WG
中科院分区：
文献类型：
--
作者：
Parr, C;Watkins, G;Jiang, WG
胎盘生长因子(PlGF)属于血管内皮生长因子(VEGF)家族，是一组对肿瘤血管生成起关键作用的血管生成因子。人们对PlGF在人类癌症中的重要性知之甚少。我们假设PlGF在乳腺癌中可能有很大的影响。这项研究检测了人类乳腺癌中PlGF水平与患者临床参数的关系。用实时定量聚合酶链式反应(Q-PCR)定量检测乳腺癌组织(n=119)和背景乳腺组织(n=33)中PlGF的表达和分布，用逆转录聚合酶链式反应(RT-PCR)定性检测多种细胞系中PlGF的表达和分布，并对患者标本进行免疫组织化学检测。与正常乳腺组织相比，乳腺癌组织中血小板生长因子的表达显著增加(P=0.028)。我们通过对患者生存状态(6年随访)的分析，证明了血浆血小板生长因子的预后价值，因为血浆血小板生长因子水平升高与复发、转移和患者死亡率显著相关(P=0.017)。我们的研究表明，PlGF在乳腺癌组织中过表达，并与患者的预后相关，可能在肿瘤的发病机制中发挥重要作用。(C)2005爱思唯尔有限公司。保留所有权利。
Placenta growth factor (PlGF) belongs to the vascular endothelial growth factor (VEGF) family, a group of angiogenic factors that are crucial for tumour angiogenesis. Very little is known about the significance of PlGF in human cancer. We hypothesise that PlGF may have a potent influence in breast cancer. This study examined PlGF levels in human breast cancer in relation to patient's clinical parameters. PlGF expression and distribution was examined quantitatively using real-time quantitative polymerase chain reaction (Q-PCR) on a cohort of human breast cancer tissue (n = 119) and background breast tissue (n = 33), qualitatively using reverse transcriptase polymerase chain reaction (RT-PCR) on a range of cell lines, and immunohistochemically on patient samples. All these techniques revealed that PlGF expression was dramatically increased (P = 0.028) in breast cancer tissues compared with normal breast tissue. We demonstrate that PlGF displays prognostic value through analysis of patient survival status (6-year follow-up), as elevated levels of PlGF were significantly associated (P = 0.017) with recurrence, metastasis and patient mortality. Our study has shown that PlGF is over-expressed in breast cancer tissues and correlates with patient prognosis, and is likely to play a major role in the pathogenesis of tumours. (c) 2005 Elsevier Ltd. All rights reserved.