Innervation of the maxillary vibrissae in mice as revealed by anterograde and retrograde tract tracing
Innervation of the maxillary vibrissae in mice as revealed by anterograde and retrograde tract tracing
复制标题
DOI：
10.1007/s00441-003-0816-z
复制
发表时间：
2004-02-01
影响因子：
3.6
通讯作者：
Hansen, LA
中科院分区：
文献类型：
--
作者：
Maklad, A;Fritzsch, B;Hansen, LA
触须是哺乳动物独特的感觉系统，其特点是丰富多样的神经支配，涉及众多感觉任务，可能存在物种特异性差异。在本研究中，结合吲哚碳花青染料（DiI和PTIR271）和共聚焦显微镜来研究小鼠三叉神经节上颌部分的触须神经支配和触须特异性感觉神经元分布。触须海绵窦（CS）的较深区域包含密集的游离神经末梢丛，可能是自主神经纤维。该窦的浅表部分显示出大量的微粒末梢。环窦区域的神经支配由默克尔末端和不同形态变异的披针形末端组成。内圆锥体区域具有自由神经末梢的圆形丛。除了证实之前通过各种其他技术和超微结构研究获得的观察结果之外，我们的研究还揭示了与之前使用大鼠进行的研究相比，其分布模式不同的更密集的末端受体末端。我们还使用来自两个附近触须的荧光示踪剂进行逆行追踪，揭示了三叉神经节中感觉神经元的分布。我们确定支配两个触毛的感觉神经元群体在很大程度上是重叠的。这表明在神经轴的不同水平上报告的振动投影的体位图并没有在神经节水平上忠实地再现。
Vibrissae are a unique sensory system of mammals that is characterized by a rich and diverse innervation involved in numerous sensory tasks with the potential for species-specific differences. In the present study, indocarbocyanine dyes (DiI and PTIR271) and confocal microscopy were combined to study the innervation of the mystacial vibrissae and vibrissa-specific sensory neuron distribution in the maxillary portion of the trigeminal ganglion of the mouse. The deeper regions of the vibrissa cavernous sinus (CS) contained a dense plexus of free nerve endings, possibly of autonomic fibers. The superficial part of this sinus displayed a massive array of corpuscular endings. Innervation in the region of the ring sinus consisted of Merkel endings and different morphological variances of lanceolate endings. The region of the inner conical body had a circular plexus of free nerve endings. In addition to confirming previous observations obtained by a variety of other techniques and ultrastructural studies, our studies revealed denser terminal receptor endings in a different distribution pattern than previously demonstrated in studies using the rat. We also revealed the distribution of sensory neurons in the trigeminal ganglion using retrograde tracing with fluorescent tracers from two nearby vibrissae. We determined that the populations of sensory neurons innervating the two vibrissae were largely overlapping. This suggests that the somatotopic maps of vibrissal projections reported at the different levels in the neuraxis are not faithfully reproduced at the level of the ganglion.