Emergence of human pandemic O25:H4-ST131 CTX-M-15 extended-spectrum-beta-lactamase-producing Escherichia coli among companion animals.
Emergence of human pandemic O25:H4-ST131 CTX-M-15 extended-spectrum-beta-lactamase-producing Escherichia coli among companion animals.
复制标题
人类大流行 O25:H4-ST131 CTX-M-15 产超广谱 β-内酰胺酶大肠杆菌在伴侣动物中出现。
DOI：
--
复制
发表时间：
2010
影响因子：
5.2
通讯作者：
S. Guenther
中科院分区：
文献类型：
--
作者：
C. Ewers;M. Grobbel;I. Stamm;P. Kopp;Ines Diehl;T. Semmler;A. Fruth;J. Beutlich;B. Guerra;L. Wieler;S. Guenther
目标
鉴于产生CTX-M-15超广谱β-内酰胺酶(ESBL)的大肠埃希菌克隆O25：H4-ST131在人类临床环境中的洲际出现，探索其在动物体内的存在，揭示其可能的存储功能，以及这组多重耐药菌株的起源和传播将是非常有意义的。
方法
在一个覆盖整个欧洲服务区的兽医诊断实验室收集了177株临床表型产ESBL大肠埃希氏菌，主要来自患有尿路感染、伤口感染和腹泻的同伴动物。筛选出分子亚型O25b和多位点序列类型131。随后对O25b-ST131分离株进行了ESBL型以及表型和基因耐药决定因素的检测。通过PFGE和毒力基因分型对菌株进行了进一步的鉴定。
结果
在177株表型产ESBL的大肠埃希菌中，有10株(5.6%)属于B2-O25b-ST131谱系，其中9株来自狗，1株来自马。其中9株具有CTX-M-15型β-内酰胺酶，1株具有SHV-12型ESBL。宏观限制性内切酶分析显示，来自5个不同欧洲国家的6株动物CTX-M-15型ESBL产生菌与1株人类对照株聚在一起，构成了一组克隆相关的菌株，相似度为87.0%。
结论
我们的发现表明，克隆相关的人类B2-O25：H4-ST131 CTX-M-15型产超广谱β-内酰胺酶大肠杆菌菌株群存在于来自不同欧洲国家的同伴动物中。这突显了这些多耐药菌株在人与动物之间跨物种传播的可能性，反之亦然。
OBJECTIVES
In view of the intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15 extended-spectrum beta-lactamase (ESBL) in human clinical settings it would be of great interest to explore its existence in animals to unravel a possible reservoir function and the origin and transmission of this group of multiresistant strains.
METHODS
A total of 177 clinical phenotypically ESBL-producing E. coli isolates, mainly obtained from companion animals with urinary tract infections, wound infections and diarrhoea, were collected in a veterinary diagnostic laboratory covering a European-wide service area. They were screened for molecular subtype O25b and multilocus sequence type 131. O25b-ST131 isolates were subsequently tested for ESBL types, and phenotypic and genotypic resistance determinants. Further characterization of the strains was performed by PFGE and virulence gene typing.
RESULTS
Ten (5.6%) of 177 phenotypically ESBL-producing E. coli isolates, nine strains from dogs and one strain from a horse, were allocated to the B2-O25b-ST131 lineage. Nine of these isolates harboured a CTX-M-15-type beta-lactamase enzyme while one strain possessed an SHV-12-type ESBL. Macrorestriction analysis revealed a cluster formation of six of the animal CTX-M-15-type ESBL-producing strains from five different European countries together with a human control strain constituting a group of clonally related strains at a similarity value of 87.0%.
CONCLUSIONS
Our findings demonstrate that the group of clonally related human B2-O25:H4-ST131 CTX-M-15-type ESBL-producing E. coli strains is present in companion animals from various European countries. This highlights the possibility of inter-species transmission of these multiresistant strains from human to animal and vice versa.