Feedback regulation of microscopes by image processing.
Feedback regulation of microscopes by image processing.
复制标题
通过图像处理对显微镜进行反馈调节。
DOI：
10.1111/dgd.12056
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Tsukada;Y.;Hashimoto;K.
计算显微镜系统正在成为成像生物现象的重要组成部分，而这种系统的发展需要设计显微镜的自动调节。自动化调节的一个重要方面是反馈调节，这是本综述的重点。随着现代显微镜系统变得越来越复杂，通常有许多必须协同工作的独立组件，计算机控制是不可避免的，因为这些多个组件的参数和定时的精确编排对于获得适当的图像至关重要。为了实现这一目标，已经开发了许多生物成像技术。在这里，我们总结了计算显微镜的基础知识，目的是建立自动调节的显微镜，重点是通过图像处理的反馈调节。这些技术允许高通量数据采集，同时监测短期和长期动态现象，这是没有自动化系统无法实现的。
Computational microscope systems are becoming a major part of imaging biological phenomena, and the development of such systems requires the design of automated regulation of microscopes. An important aspect of automated regulation is feedback regulation, which is the focus of this review. As modern microscope systems become more complex, often with many independent components that must work together, computer control is inevitable since the exact orchestration of parameters and timings for these multiple components is critical to acquire proper images. A number of techniques have been developed for biological imaging to accomplish this. Here, we summarize the basics of computational microscopy for the purpose of building automatically regulated microscopes focus on feedback regulation by image processing. These techniques allow high throughput data acquisition while monitoring both short‐ and long‐term dynamic phenomena, which cannot be achieved without an automated system.