BAG-OF-MARBLES - A DROSOPHILA GENE REQUIRED TO INITIATE BOTH MALE AND FEMALE GAMETOGENESIS
BAG-OF-MARBLES - A DROSOPHILA GENE REQUIRED TO INITIATE BOTH MALE AND FEMALE GAMETOGENESIS
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DOI：
10.1101/gad.4.12b.2242
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发表时间：
1990-12-01
影响因子：
10.5
通讯作者：
SPRADLING, AC
中科院分区：
文献类型：
--
作者：
MCKEARIN, DM;SPRADLING, AC
在果蝇中，雄性和雌性配子开始发育时，干细胞分裂产生一个囊肿前体。 随后，四个特殊的分裂产生了一个由16个相互连接的囊细胞组成的簇，这些囊细胞发育成一个卵子或64个精子。 我们发现并鉴定了一种基因，大理石袋（bam），它能破坏两性的囊肿形成。 一个明显的无效突变会导致异常的囊肿形成，其中含有过量的不能分化成配子的细胞。 BAM功能存在于编码单个442个氨基酸的蛋白质的简单的2.2-kb转录单位内，该蛋白质显示出与卵巢肿瘤基因产物的相似性。 bam RNA在女性成囊细胞中的特异性表达表明，它可能参与了囊细胞分裂过程中发生的特定细胞周期改变。
In Drosophila, male and female gametes begin development when a stem cell divides to produce a cyst precursor. Subsequently, four special divisions give rise to a cluster of 16 interconnected cystocytes that develop into a single egg or 64 sperm. We identified and characterized a gene, bag-of-marbles (bam), that disrupts cyst formation in both sexes. An apparent null mutation causes abnormal cysts to form containing an excess number of cells that cannot differentiate into gametes. bam function resides within a simple 2.2-kb transcription unit encoding a single 442-amino-acid protein that shows similarity to the product of the ovarian tumor gene. The specific expression of bam RNA within female cystoblasts suggested that it might be involved in the specific cell-cycle alterations that occur during cystocyte divisions.