Fine structure of gonadotrophs and prolactin producing cells in the rat pars distalis in organ culture.
Fine structure of gonadotrophs and prolactin producing cells in the rat pars distalis in organ culture.
复制标题
器官培养中大鼠远端促性腺激素和催乳素产生细胞的精细结构。
作者：
T. G. Baker;B. A. Young
描述了一种技术，允许维持长达8天的器官培养外植大鼠脑垂体。电镜研究表明，培养的腺体（无论是否用促性腺激素释放激素（GnRH）处理）在外观上几乎与对照组相同，尽管星状（滤泡）细胞似乎在体外被消除了。男性和女性脑垂体中的促性腺激素和催乳素分泌细胞在体内和体外的外观与其他作者描述的类似细胞相似。有一些证据表明，当外植体用GnRH处理时，这些细胞类型（特别是促性腺细胞）的分泌活性增加。因此，目前的技术为研究GnRH和类固醇激素对体外FSH和LH释放和合成的影响提供了一个合适和有效的“模型”。
A technique is described which permits the maintenance for up to 8 days in organ culture of explanted rat pituitary glands. Electron microscope studies showed that the cultured glands (whether treated with gonadotrophin-releasing hormone (GnRH) or not) were almost identical to controls in appearance, although the stellate (follicular) cells seemingly were eliminated in vitro. Gonadotrophs and prolactin-secreting cells in male and female pituitary glands were similar in appearance both in vivo and in vitro to comparable cells described by other authors. There was some evidence for an increase in the secretory activity of these cell types (particularly gonadotrophs) when the explants were treated with GnRH. The present technique thus provides a suitable and valid 'model' with which to study the effects of GnRH and steroid hormones on the release and synthesis of FSH and LH in vitro.