Centromeric DNA hypomethylation as an epigenetic signature discriminates between germ and somatic cell lineages
Centromeric DNA hypomethylation as an epigenetic signature discriminates between germ and somatic cell lineages
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DOI：
10.1016/j.ydbio.2007.09.041
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发表时间：
2007-12-01
影响因子：
2.7
通讯作者：
Baba, Tadashi
中科院分区：
文献类型：
--
作者：
Yamagata, Kazuo;Yamazaki, Taiga;Baba, Tadashi
生殖细胞具有与体细胞明显不同的独特特征。这两种细胞谱系之间的功能分歧已被假定为表观遗传机制的结果。在这里，我们表明，在男性和女性生殖细胞的染色体中心和臂间（C/P）区域是专门的DNA低甲基化，尽管在体细胞的高甲基化状态。在多能生殖系干细胞中，C/P区最初是低甲基化的，然后在体外分化为体细胞谱系的过程中转变为高甲基化状态。此外，体细胞来源的核移植胚胎在整个植入前发育过程中保持了体细胞类型的高甲基化模式。这些结果意味着生殖细胞谱系的身份可能是由C/P区的低甲基化状态作为表观遗传标记来保证的。(c)2007爱思唯尔公司All rights reserved.
Germ cells have unique features strikingly distinguishable from somatic cells. The functional divergence between these two cell lineages has been postulated to result from epigenetic mechanisms. Here we show that the chromosomal centric and pericentric (C/P) regions in male and female germline cells are specifically DNA-hypomethylated, despite the hypermethylation status in somatic cells. In multipotent germline stem cells, the C/P region was initially hypomethylated and then shifted to the hypermethylation status during differentiation into somatic lineage in vitro. Moreover, the somatic-type hypermethylation pattern was maintained in the somatic cell-derived nuclear transfer embryos throughout preimplantation development. These results imply that the identity of germ cell lineage may be warranted by the hypomethylation status of the C/P region as an epigenetic signature. (c) 2007 Elsevier Inc. All rights reserved.