Co-induction of activity-dependent genes in songbirds.
Co-induction of activity-dependent genes in songbirds.
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鸣禽活动依赖性基因的共诱导。
DOI：
10.1111/j.1460-9568.2005.04369.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Mello,ClaudioV
中科院分区：
文献类型：
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作者：
Velho,TarcisoAF;Pinaud,Raphael;Rodrigues,PauloV;Mello,ClaudioV
鸣禽的歌唱行为诱导了参与歌唱知觉处理、产生和学习的大脑区域活动依赖基因的表达。这种基因组反应被认为代表了神经元激活和鸣禽大脑中歌曲处理电路的长期变化之间的联系。在这里，我们证明了Arc，一个活性调节基因，其产物具有树突定位，并与突触可塑性相关，在斑马雀的大脑中被歌声迅速诱导。我们发现，在歌曲听觉刺激的背景下，Arcis的表达在几个端脑听区中被诱导，最显著的是在尾内侧的中脑和中脑；而在歌唱的背景下，Arcis的表达也在歌曲控制区，即专有名称的HVC核、延髓的粗壮核和nidopallium的界面核中诱导。我们还发现，SONG诱导的Arc表达与转录调控因子zenkA和c-fos在细胞水平上是共定位的，并且这三个基因的SONG诱导依赖于丝裂原活化蛋白激酶信号通路的激活。这些发现为Arin参与大脑对鸟鸣的反应提供了证据。他们还证明，代表不同基因组和细胞调控程序的基因，即早期效应器和转录因子，在同一神经细胞中由自然获得的刺激共同激活。
Song behavior in songbirds induces the expression of activity‐dependent genes in brain areas involved in perceptual processing, production and learning of song. This genomic response is thought to represent a link between neuronal activation and long‐term changes in song‐processing circuits of the songbird brain. Here we demonstrate thatArc, an activity‐regulated gene whose product has dendritic localization and is associated with synaptic plasticity, is rapidly induced by song in the brain of zebra finches. We show that, in the context of song auditory stimulation,Arcexpression is induced in several telencephalic auditory areas, most prominently the caudomedial nidopallium and mesopallium, whereas in the context of singing,Arcis also induced in song control areas, namely nucleus HVC, used as a proper name, the robust nucleus of the arcopallium and the interface nucleus of the nidopallium. We also show that song‐inducedArcexpression co‐localizes at the cellular level with those of the transcriptional regulatorszenkandc‐fos, and that the song induction of these three genes is dependent on activation of the mitogen‐activated protein kinase signaling pathway. These findings provide evidence for an involvement ofArcin the brain's response to birdsong. They also demonstrate that genes representing distinct genomic and cellular regulatory programs, namely early effectors and transcription factors, are co‐activated in the same neuronal cells by a naturally learned stimulus.