Eag1, Eag2, and SK3 potassium channel expression in the rat hippocampus after global transient brain ischemia
Eag1, Eag2, and SK3 potassium channel expression in the rat hippocampus after global transient brain ischemia
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全脑短暂性脑缺血后大鼠海马 Eag1、Eag2 和 SK3 钾通道表达
DOI：
10.1002/jnr.22772
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发表时间：
2012
影响因子：
4.2
通讯作者：
E. A. D. Bel
中科院分区：
文献类型：
--
作者：
R. M. W. D. Oliveira;Sabine Martin;C. L. D. Oliveira;Humberto Milani;A. P. Schiavon;S. R. Joca;Luis A. Pardo;Walter Stühmer;E. A. D. Bel
短暂性脑缺血导致海马神经元延迟死亡，这与海马依赖性脑功能（如情绪、学习和记忆）的损伤有关。我们研究了瞬时脑缺血后大鼠海马中电压依赖性Kcnh1和Kcnh5， ether - go - go相关Eag1和Eag2 （KV10.1和KV10.2）以及小电导钙激活的SK3 (KCa2.3, Kcnn3) K+通道的表达。我们通过评估动物在升高+迷宫和下降抑制性回避任务中的表现来测试这些通道的表达是否与行为改变有关。在短暂性全脑缺血后7天或28天，对一组大鼠进行双侧海马解剖，并测定mRNA水平。短暂性全脑缺血后7天，大鼠表现出焦虑样行为和记忆障碍的减少。缺血后28天焦虑水平升高。短暂性全脑缺血7天后，海马组织中Eag2 mRNA表达下调，而Eag1和SK3 mRNA表达保持不变。这是第一个证明短暂性脑缺血会暂时改变Eag2的实验证据。与缺血后7或28天的假对照动物相比，CA1和CA2， CA3和CA4海马亚区中完整出现的锥体神经元数量显著减少，具有统计学意义。大鼠海马mRNA表达。本研究结果为研究Eag2通道在中枢神经系统中的生理作用提供了进一步的信息。©2011 Wiley期刊公司
Transient global brain ischemia causes delayed neuronal death in the hippocampus that has been associated with impairments in hippocampus‐dependent brain function, such as mood, learning, and memory. We investigated the expression of voltage‐dependent Kcnh1 and Kcnh5, ether à go‐go‐related Eag1 and Eag2 (KV10.1 and KV10.2), and small‐conductance calcium‐activated SK3 (KCa2.3, Kcnn3) K+ channels in the hippocampus in rats after transient global brain ischemia. We tested whether the expression of these channels is associated with behavioral changes by evaluating the animals in the elevated plus maze and step‐down inhibitory avoidance task. Seven or tweny‐eight days after transient global brain ischemia, one group of rats had the hippocampus bilaterally dissected, and mRNA levels were determined. Seven days after transient global brain ischemia, the rats exhibited a decrease in anxiety‐like behavior and memory impairments. An increase in anxiety levels was detected 28 days after ischemia. Eag2 mRNA downregulation was observed in the hippocampus 7 days after transient global brain ischemia, whereas Eag1 and SK3 mRNA expression remained unaltered. This is the first experimental evidence that transient global brain ischemia temporarily alters Eag2. The number of intact‐appearing pyramidal neurons was substantially decreased in CA1 and statistically measurable in CA2, CA3, and CA4 hippocampal subfields compared with sham control animals 7 or 28 days after ischemia. mRNA expression in the rat hippocampus. The present results provide further information for the characterization of the physiological role of Eag2 channels in the central nervous system. © 2011 Wiley Periodicals, Inc.
DOI：
10.1073/pnas.91.21.10044
发表时间：
1994-10-11
影响因子：
11.1
作者：
GRIFFITH, LC;WANG, J;GREENSPAN, RJ
通讯作者：
GREENSPAN, RJ