Keratinocyte growth factor prevents intra-alveolar oedema in experimental lung isografts
Keratinocyte growth factor prevents intra-alveolar oedema in experimental lung isografts
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DOI：
10.1183/09031936.00011707
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发表时间：
2008-01-01
影响因子：
24.3
通讯作者：
Grau, V.
中科院分区：
文献类型：
--
作者：
Sadovski, J.;Kuchenbuch, T.;Grau, V.
Primary graft dysfunction, characterised by intra-alveolar oedema, is a major obstacle in pulmonary transplantation. The present study evaluates the potential of keratinocyte growth factor (palmiferin; Delta N23-KGF) for the prevention of oedema in lung transplants. Intratracheal instillation of 5 mg center dot kg(-1) Delta N23-KGF was performed in Lewis rats on days 3 and 2 before explantation. Control animals obtained an equivalent volume of vehicle. Left lungs were isogeneically transplanted and the graft recipients were sacrificed 1 day later for stereological analysis of intra-alveolar oedema and bronchoalveolar lavage. The total protein and phospholipid content, as well as surfactant proteins, were measured. Surfactant activity was analysed with a pulsating bubble surfactometer. In grafts from control treated donors, the fraction of intra-alveolar oedema amounted to 3.4 +/- 1.1% of the total parenchymal volume. Treatment of donor lungs with Delta N23-KGF reduced oedema to a fraction of 1.6 +/- 0.8%. In the lavage fluid of pulmonary grafts from Delta N23-KGF-treated donors, the total protein content was decreased compared with vehicle-treated lung transplants, whereas phospholipids did not differ. The protein fraction contained increased amounts of surfactant protein-C after Delta N23-KGF treatment and surfactant function was improved. Treatment of donor lungs with palifermin protects against intra-alveolar oedema formation upon transplantation. This effect appears to be mediated by an improved surfactant homeostasis.
