Microbial community and physicochemical analysis of an industrial waste gas biofilter and design of 16S rRNA-targeting oligonucleotide probes
Microbial community and physicochemical analysis of an industrial waste gas biofilter and design of 16S rRNA-targeting oligonucleotide probes
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DOI：
10.1046/j.1462-2920.2003.00397.x
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发表时间：
2003-03-01
影响因子：
5.1
通讯作者：
Lipski, A
中科院分区：
文献类型：
--
作者：
Friedrich, U;Van Langenhove, H;Lipski, A
研究了一种用于动物屠宰厂废气治理的工业生物滤池的微生物群落结构、理化性质及其相互关系。荧光原位杂交（FISH）成功地结合数字图像分析，研究了微生物群落的组成。几个新的核酸探针的设计和建立的基础上公布的16 S rDNA序列和检索的生物过滤器的生物量的调查。细菌检出率随时间和滤床深度变化很大，相对于DAPI计数，在27.2%至88.1%之间。总的来说，β-变形菌门的成员其次是放线菌门、α-变形菌门、噬细胞-黄细菌门、厚壁菌门和γ-变形菌门是最丰富的类群。在低于门水平的组中，产碱菌/博德特氏菌谱系的成员平均是最丰富的组，占DAPI染色细胞的8.5%。而社区组成一般没有表现出垂直梯度，较低的50厘米的生物过滤器被证明是最活跃的部分，如2-和3-甲基丁醛，2-甲基丙醛，和己醛的降解。在向上流动方向上操作的过滤床的该区域降解了约80%的这些化合物。二甲基二硫化物是最常见的还原硫化合物。微生物与废气参数的统计分析通常显示两者之间只有微弱或不显著的相关性。对这一发现的可能解释进行了讨论。
A study was conducted to investigate the microbial community structure, the physicochemical properties, and the relationships between these parameters of a full-scale industrial biofilter used for waste gas abatement in an animal-rendering plant. Fluorescence in situ hybridization (FISH) was successfully combined with digital image analysis to study the composition of the microbial community. Several new nucleic acid probes were designed and established based on published 16S rDNA sequences and on ones retrieved from the biomass of the biofilter under investigation. Bacterial detection rates varied greatly over time and filterbed depth between 27.2% and 88.1% relative to DAPI counts. Overall, members of the Betaproteobacteria followed by Actinobacteria , Alphaproteobacteria , Cytophaga-Flavobacteria , Firmicutes and Gammaproteobacteria were the most abundant groups. Among the groups below phylum level, members of the Alcaligenes/Bordetella lineage were on average the most abundant group accounting for up to 8.5% of DAPI-stained cells. Whereas the community composition generally showed no vertical gradient, the lower 50 cm of the biofilter proved to be the most active part for the degradation of aldehydes such as 2- and 3-methylbutanal, 2-methylpropanal, and hexanal. This zone of the filterbed being operated in up-flow direction degraded about 80% of these compounds. Dimethyldisulphide was the most common reduced sulphur compound. Statistical analysis of microbial versus waste gas parameters generally revealed only weak or non-significant correlations between the two. Possible explanations for this finding are discussed.