Protective Effect of Ethyl Acetate Fraction of Stereospermum Suaveolens Against Hepatic Oxidative Stress in STZ Diabetic Rats.
Protective Effect of Ethyl Acetate Fraction of Stereospermum Suaveolens Against Hepatic Oxidative Stress in STZ Diabetic Rats.
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DOI：
10.4103/2225-4110.114904
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发表时间：
2013-07
影响因子：
4.5
通讯作者：
Chatterjee TK
中科院分区：
文献类型：
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作者：
Balasubramanian T;Senthilkumar GP;Karthikeyan M;Chatterjee TK
Stereospermum suaveolens是印度南部地区治疗糖尿病和肝脏疾病的民间疗法。本研究采用离体细胞培养法，观察了S.在链脲佐菌素（STZ）诱导的糖尿病大鼠中评价甜菊素对肝脏氧化应激的影响，持续14天。将乙酸乙酯级分以200和400 mg/kg的剂量经口给予STZ糖尿病大鼠。采用葡萄糖氧化酶法测定血糖水平。为了确定肝保护活性，在乙酸乙酯级分处理的糖尿病大鼠中评估血清生物标志物酶如天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和血清碱性磷酸酶（SALP）水平的变化，并与糖尿病对照大鼠中的水平进行比较。此外，使用各种肝脏参数，如硫代巴比妥酸反应物质（TBARS），还原型谷胱甘肽（GSH），超氧化物歧化酶（SOD），和过氧化氢酶（CAT）的乙酸乙酯馏分的抗氧化活性进行了评价。结果发现，给予乙酸乙酯部分（200和400 mg/kg）可显著（P < 0.001）降低糖尿病大鼠的空腹血糖水平、TBARS、胆红素、AST、ALT和SALP，同时升高GSH水平、SOD和CAT活性。组织病理学研究也显示了乙酸乙酯部位对糖尿病大鼠肝组织的保护作用。结果表明，S. suaveolens调节酶和非酶抗氧化剂的活性，并增强对STZ诱导的糖尿病大鼠肝脏氧化应激的防御。
Stereospermum suaveolens is a folk remedy for the treatment of diabetes and liver disorders in southern parts of India. In the present study, the protective effect of the ethyl acetate fraction of ethanol extract from S. suaveolens against hepatic oxidative stress was evaluated in streptozotocin (STZ)-induced diabetic rats for 14 days. The ethyl acetate fraction was administered orally to the STZ diabetic rats at the doses of 200 and 400 mg/kg. Blood glucose level was measured according to glucose oxidase method. In order to determine hepatoprotective activity, changes in the levels of serum biomarker enzymes such as aspartate transaminase (AST), alanine transaminase (ALT), and serum alkaline phosphatase (SALP) were assessed in the ethyl acetate fraction treated diabetic rats and were compared with the levels in diabetic control rats. In addition, the antioxidant activity of ethyl acetate fraction was evaluated using various hepatic parameters such as thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT). It was found that administration of ethyl acetate fraction (200 and 400 mg/kg) produced a significant (P < 0.001) fall in fasting blood glucose level, TBARS, bilirubin, AST, ALT, and SALP, while elevating the GSH levels, and SOD and CAT activities in diabetic rats. Histopathologic studies also revealed the protective effect of ethyl acetate fraction on the liver tissues of diabetic rats. It was concluded from this study that the ethyl acetate fraction from ethanol extract of S. suaveolens modulates the activity of enzymatic and nonenzymatic antioxidants and enhances the defense against hepatic oxidative stress in STZ-induced diabetic rats.