An in silico immunological approach for prediction of CD8+T cell epitopes of Leishmania major proteins in susceptible BALB/c and resistant C57BL/6 murine models of infection
An in silico immunological approach for prediction of CD8+T cell epitopes of Leishmania major proteins in susceptible BALB/c and resistant C57BL/6 murine models of infection
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DOI：
10.1016/j.meegid.2008.02.011
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发表时间：
2009-05-01
影响因子：
3.2
通讯作者：
Laouini, D.
中科院分区：
文献类型：
--
作者：
Guerfali, F. Z.;Ben-Abdallah, H.;Laouini, D.
已经确定，在C57 BL/6抗性小鼠品系中，MHC II类限制性CD 4 T细胞在针对利什曼原虫（L）的免疫力发展过程中占主导地位。然而，与许多先前的观察结果一致，这些观察结果表明特定的COB T细胞在人类自然感染或疫苗接种期间被引发，最近从易感BALB/c小鼠获得的大量证据表明，c小鼠感染硕大利什曼原虫模型表明，CD 8 T细胞参与皮肤利什曼病的发病机制和免疫。整个L。主要预测蛋白质组，使用几种公共计算系统预测肽与BALB/c和C57 BL/6小鼠中所有MHC（组织相容性复合物-2）分子的结合（Syfpeithi、Rankpep、PREDBALB/c和Bimas）。然后使用BLAST算法分析预测与不同H2分子结合的肽与迄今为止注释的任何鼠蛋白的同源性。通过不同的预测系统定义每个H2分子的选定肽组，并相互比较。我们的发现说明了一种混合免疫计算的方法如何可能是有用的生物学家靶向一个在硅片上的一组选定的蛋白质，以确定潜在的候选抗原的实验疫苗接种具有更高的准确性，以及减少数量的T细胞抗原。(C)2008 Elsevier B. V.保留所有权利。
It is well established that MHC class II restricted-CD4 T cells are dominant during the development of immunity against Leishmania (L) in the C57BL/6-resistant mouse strain. However and in agreement with a number of previous observations indicating that specific COB T cells are primed during natural infection or vaccination in humans, a great deal of evidence obtained recently with the susceptible BALB/c murine model of infection by Leishmania major indicates that CD8 T cells participate in both pathogenesis and immunity to cutaneous leishmaniasis.Our goal herein was to identify in silica all parasitic peptides present in the whole L. major predicted proteome, using several public computational systems for the prediction of peptide binding to all MHC (histocompatibility complex-2) molecules in BALB/c and C57BL/6 mice (Syfpeithi, Rankpep, PREDBALB/c and Bimas). Peptides that were predicted to bind to different H2 molecules were then analysed for their homology with any of the murine proteins annotated so far, using the BLAST algorithm. Sets of selected peptides for each H2 molecule were defined by different prediction systems and compared to each other.Surprisingly, the results showed that a higher number of L. major peptides were predicted to bind H2 BALB/c molecules and very few or none to bind H2 C57BL/6 molecules.Our finding illustrates how a hybrid immuno-computational approach may be useful for biologists to target an in silico set of selected proteins to define potential candidate antigens for experimental vaccination with greater accuracy as well as a reduced number of T cell antigens. (C) 2008 Elsevier B.V. All rights reserved.