Human lymphocyte response to phytomitogens in vitro: normal, agammaglobulinemic and paraproteinemic individuals.
Human lymphocyte response to phytomitogens in vitro: normal, agammaglobulinemic and paraproteinemic individuals.
复制标题
人类淋巴细胞对体外植物丝裂原的反应：正常、无丙种球蛋白血症和副蛋白血症个体。
DOI：
10.4049/jimmunol.103.6.1185
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发表时间：
1969
影响因子：
4.4
通讯作者：
H. Fudenberg
中科院分区：
文献类型：
--
作者：
Steven D. Douglas;R. Kamin;H. Fudenberg
对四种植物有丝分裂原在10天内刺激正常人淋巴细胞时胸腺嘧啶掺入的时间过程的研究表明，在反应谱上存在一些差异。植物血凝素（PHA）、蜡豆糖蛋白（wax bean glycoprotein）和豆蛋白A （concanavalin A）对培养物的响应在第3或5天达到峰值，而美洲商陆丝裂原（PWM）对培养物的响应在第5或7天达到较低的峰值。PHA、蜡豆糖蛋白和PWM刺激的培养在第7天和第10天继续显示显著的胸苷结合，尽管Con A的反应在7天后恢复到基线。
Studies of the time course of thymidine incorporation during stimulation of lymphocytes from normal individuals with four plant mitogens over a 10-day period demonstrate several differences in response profiles. Whereas the peak responses for cultures stimulated with phytohemagglutinin (PHA), wax bean glycoprotein and concanavalin A (Con A) occurred at 3 or 5 days, the lower peak response to pokeweed mitogen (PWM) was observed at 5 or 7 days. Cultures stimulated with PHA, wax bean glycoprotein and PWM continued to show significant thymidine incorporation at 7 and 10 days, although the response to Con A had returned to baseline after 7 days.
Lymphocytes from eight adult “acquired” agammaglobulinemic patients and their four available parents had a diminished thymidine incorporation in 3-day cultures using each of the four mitogens (p < 0.001). A late response was observed with PHA or wax bean stimulation at 5 to 7 days, comparable to controls at 3 days. With Con A, lymphocytes from both the “acquired” agammaglobulinemic patients and their parents had a diminished incorporation throughout the 10-day period (p < 0.001). Stimulation with PWM distinguished the lymphocytes of the patients from their heterozygous parents; at 5 to 7 days the parents had a peak comparable to controls, whereas the “acquired” agammaglobulinemics had a diminished peak (p < 0.001) at 10 days.
Lymphocytes from patients with multiple myeloma and Waldenström's macroglobulinemia had diminished thymidine incorporation in response to Con A during a 10-day incubation period. These patients had normal or late responses comparable in magnitude to controls with PHA, wax bean glycoprotein and PWM.
These observations suggest that plant mitogens have selective and specific actions on lymphocyte subpopulations. Differences in the time course and magnitude of lymphocyte response to plant mitogens may reflect different circulating subpopulations of cells or alterations in the total number of cells responsive to mitogen. Detailed time studies using several mitogenic agents represent a possible approach to the assessment of lymphocyte function in immunologic diseases.