Evidence in rat and mouse liver for temporal control of two forms of cytochrome P-450 inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Evidence in rat and mouse liver for temporal control of two forms of cytochrome P-450 inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
复制标题
大鼠和小鼠肝脏中 2,3,7,8-四氯二苯并-对二恶英诱导的两种形式的细胞色素 P-450 的时间控制的证据。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
D. Nebert
中科院分区：
文献类型：
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作者：
T. Guenthner;D. Nebert
在围产期大鼠或小鼠的肝脏中，2，3，7，8-四氯二苯并-对二恶英诱导的芳烃羟基酶与细胞色素P-450总含量的比值在妊娠后18天或19天至22天期间下降，而2，3，7，8-四氯二苯并-对二恶英诱导的乙酰苯胺酶与细胞色素P-450总含量的比值在妊娠后18~19天至22天升高。可诱导芳烃羟基酶的个体发生与56000-MR电泳带的增加很好地对应；我们认为该带代表细胞色素P1-450亚单位。可诱导的乙酰苯胺4-羟基酶的后期表达与2，3，7，8-四氯二苯并对二恶英引起的54000-MR电泳带的尺寸增加和总微粒体还原细胞色素P-450的SORET峰2-3-3 nm亚色移密切相关。CO情结。我们认为该条带代表细胞色素P-448亚基。该实验室以前的工作表明，这种发育差异在兔肝脏中是几周的分离，而本报告中显示的大鼠或小鼠肝脏的发育差异是几天的分离。因此，这里的数据为在啮齿类动物中时间控制由ah基因调控的不同结构基因产物的表达提供了证据。
In the liver of perinatal rats or mice, the ratio of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced aryl hydrocarbon hydroxylase to total cytochrome P-450 content decreases, whereas the ratio of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced acetanilide 4-hydroxylase to total cytochrome P-450 content increases, between 18 or 19 days and 22 days following conception. The ontogenesis of inducible aryl hydrocarbon hydroxylase corresponds well with increases in a 56000-Mr electrophoretic band; we suggest this band represents the cytochrome P1-450 subunit. The later temporal expression of inducible acetanilide 4-hydroxylase closely parallels 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced increases in size of a 54000-Mr electrophoretic band and a 2--3-nm hypsochromic shift in the Soret peak of the total microsomal reduced cytochrome P-450 . CO complex. We suggest this band represents the cytochrome P-448 subunit. Previous work from this laboratory has shown that this developmental difference is separated by several weeks in rabbit liver, as compared with several day's separation shown in this report with rat or mouse liver. The data here therefore provide evidence in the rodent for temporal control of the expression of different structural gene products regulated by the Ah locus.