Comparative Cell Wall Analyses of Morphological Forms Within the Genus Actinomyces
Comparative Cell Wall Analyses of Morphological Forms Within the Genus Actinomyces
复制标题
DOI：
10.1128/jb.94.4.875-883.1967
复制
发表时间：
1967-10
影响因子：
3.2
通讯作者：
L. Pine;C. J. Boone
中科院分区：
文献类型：
--
作者：
L. Pine;C. J. Boone
对牛放线菌和放线菌菌丝体和光滑体的细胞壁进行了比较分析。israelii的细胞壁分析，以确定发生在细胞壁组成的变化，同时在形态学上的变化，并评估细胞壁分析作为分类鉴定放线菌属内的标准。蜘蛛型A. Boyis具有很少或没有天冬氨酸和高浓度的己糖胺;光滑形式的细胞壁具有高天冬氨酸含量和低浓度的己糖胺。正如先前报道的，这两种形式都含有大量的谷氨酸、丙氨酸和赖氨酸。一株放线菌（Actinomyces），以前被鉴定为A.根据形态学和好氧生长特性，发现内氏藻具有A.以色列。当从放线菌维持肉汤转移到巯基乙酸盐肉汤时，该菌株的细胞通过持续孵育从菌丝体形式通过瞬时丝状形态变成白喉状。同时，谷氨酸相对于丙氨酸的浓度降低，氨基己糖含量增加。种内形态变异。israelii和A.牛的死亡与它们细胞壁的任何相互化学变化无关。
Comparative cell wall analyses were made of mycelial and smooth forms of Actinomyces bovis and A. israelii to determine the changes which occur in the cell wall composition concurrent with a change in morphology, and to evaluate cell wall analyses as a criterion for taxonomic identification within the genus Actinomyces. Cell walls of the spider forms of A. boyis had little or no aspartic acid and a high hexosamine concentration; cell walls of the smooth forms had a high aspartic acid content and low concentrations of hexosamine. Both forms had large amounts of glutamic acid, alanine, and lysine, as previously reported. A strain of Actinomyces, previously identified as A. naeslundii on the basis of morphology and aerobic growth characteristics, was found to have the basic cell wall composition of A. israelii. When transferred from the Actinomyces maintenance broth to a thioglycolate broth, the cells of this strain passed from a mycelial form through a transient filamentous morphology to become diphtheroidal with continued incubation. Concomitantly, the concentrations of glutamic acid relative to alanine decreased, and the hexosamine content increased. Variation in morphology within the species A. israelii and A. bovis could not be related to any mutual chemical change of their cell walls.