The shoot controls zeatin riboside export from pea roots. Evidence from the branching mutant rms4
The shoot controls zeatin riboside export from pea roots. Evidence from the branching mutant rms4
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DOI：
10.1046/j.1365-313x.1997.11020339.x
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发表时间：
1997-02-01
期刊：
影响因子：
7.2
通讯作者：
Rameau, C
中科院分区：
文献类型：
--
作者：
Beveridge, CA;Murfet, IC;Rameau, C
以豌豆（Pisum sativum L.）的rms4突变体为研究对象，进行了嫁接研究和根木质部汁液的细胞分裂素分析，证明至少对豌豆来说，茎部可以改变细胞分裂素从根的输入。rms4突变导致根木质部汁液中玉米蛋白核苷（ZR）浓度显著降低（降低40倍），其表型为茎部分枝增加。野生型（WT）和rms4植株之间的嫁接结果表明，根木质部汁液中细胞分裂素的浓度几乎完全由茎的基因型决定。WT接穗使rms4突变体根系液中细胞分裂素浓度正常化，而突变体接穗使WT根系表现出与自嫁接突变体植株相似的行为。豌豆rms4芽导致细胞分裂素从根部出口下调的机制目前尚不清楚。然而，我们的数据提供了证据，表明茎部向根传递信号，从而控制根中细胞分裂素生物合成的调节过程。
The rms4 mutant of pea (Pisum sativum L.) was used in grafting studies and cytokinin analyses of the root xylem sap to provide evidence that, at least for pea, the shoot can modify the import of cytokinins from the root. The rms4 mutation, which confers a phenotype with increased branching in the shoot, causes a very substantial decrease (down to 40-fold less) in the concentration of zeatin riboside (ZR) in the xylem sap of the roots. Results from grafts between wild-type (WT) and rms4 plants indicate that the concentration of cytokinins in the xylem sap of the roots is determined almost entirely by the genotype of the shoot. WT scions normalize the cytokinin concentration in the sap of rms4 mutant roots, whereas mutant scions cause WT roots to behave like those of self-grafted mutant plants. The mechanism whereby rms4 shoots of pea cause a down-regulation in the export of cytokinins from the roots is unknown at this time. However, our data provide evidence that the shoot transmits a signal to the roots and thereby controls processes involved in the regulation of cytokinin biosynthesis in the root.