Development of a rubella virus DNA vaccine.
Development of a rubella virus DNA vaccine.
复制标题
风疹病毒 DNA 疫苗的开发。
DOI：
10.1016/s0264-410x(98)00371-5
复制
发表时间：
1999
期刊：
影响因子：
5.5
通讯作者：
Frey,TK
中科院分区：
文献类型：
--
作者：
Pougatcheva,SO;Abernathy,ES;Vzorov,AN;Compans,RW;Frey,TK
利用风疹病毒基因组RNA cDNA克隆构建了两种风疹病毒DNA疫苗，其中一种包含所有三种病毒粒子蛋白（C、E2和E1）的编码序列，另一种包含两种包膜糖蛋白（E2和E1）的编码序列。当通过基因枪传递给小鼠免疫时，两种构建物诱导的抗体反应滴度与风疹病毒诱导的抗体反应滴度相当，并持续至少7个月。初次注射后4周进行加强注射，抗体滴度增加30倍以上。在注射DNA疫苗的小鼠中，抗体反应主要针对E1糖蛋白，在注射风疹病毒的小鼠中也是如此，并且检测到中和活性。因此，这些DNA疫苗是可在特殊情况下使用的非复制性风疹病毒疫苗的原型。
Two rubella virus DNA vaccines were constructed from a cDNA clone of the rubella virus genomic RNA, one which contained the coding sequences for all three virion proteins (C, E2 and E1) and one which contained the two envelope glycoproteins (E2 and E1). When used to immunize mice via gene gun delivery, both constructs induced an antibody response of equivalent titer to that induced by rubella virus that persisted for at least seven months. A booster injection given four weeks after the initial injection increased antibody titers by over thirty-fold. The antibody response in DNA vaccine-injected mice was directed primarily against the E1 glycoprotein, as was the case in rubella virus-injected mice, and neutralizing activity was detected. These DNA vaccines are thus prototypes for a nonreplicating rubella virus vaccine that could be used in specialized circumstances.