Clinical evaluation of chemosensitivity testing for patients with colorectal cancer using MTT assay
Clinical evaluation of chemosensitivity testing for patients with colorectal cancer using MTT assay
复制标题
MTT法对结直肠癌患者化疗敏感性检测的临床评价
DOI：
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复制
发表时间：
1996
影响因子：
3.9
通讯作者：
K. Ishimoto
中科院分区：
文献类型：
--
作者：
H. Yamaue;H. Tanimura;M. Nakamori;K. Noguchi;M. Iwahashi;M. Tani;T. Hotta;K. Murakami;K. Ishimoto
结直肠癌是化疗最难治疗的肿瘤之一。对于对抗癌药物敏感性较低的结直肠癌患者，应进行化疗药物敏感性试验，使化疗方案个体化。本研究旨在使用高度纯化的肿瘤细胞确定新鲜人结直肠癌的化疗敏感性，以及这种敏感性与临床反应的相关性。方法：我们用MTT法测定了93例新鲜人大肠癌对顺铂、丝裂霉素C、阿霉素和5-氟尿嘧啶的体外药物敏感性，并根据MTT法的结果进行化疗。结果：顺铂对肿瘤细胞的抑制率高于其他药物。15例具有可评估病变的患者根据MTT测定结果接受化疗。15例患者中有5例获得临床应答，应答者的顺铂抑制率高于无应答者。结论：以MTT法为指标的化疗对结直肠癌有较好的疗效。
PURPOSE: Colorectal cancer is one of the tumors most refractory to treatment by chemotherapy. The chemosensitivity test should be performed to individualize the chemotherapy for patients with colorectal cancer, which is less sensitive for anticancer drugs. The present study was designed to determine the chemosensitivity in fresh human colorectal cancer, using highly purified tumor cells, and the correlation of this sensitivity with clinical response. METHODS: We determined the chemosensitivity for cisplatin, mitomycin C, adriamycin, and 5-fluorouracilin vitroin 93 fresh human colorectal cancers using the MTT assay and performed chemotherapy according to results of the MTT assay. RESULTS: Inhibition rate of tumor cells for cisplatin was higher than those for other drugs. Fifteen patients who have evaluable lesions received chemotherapy according to results of the MTT assay. Clinical responses were obtained in 5 of 15 patients, and the inhibition rate for cisplatin was higher in responders than in nonresponders. CONCLUSIONS: It is suggested that the chemotherapy according to results of the MTT assay is effective in patients with colorectal cancer.
人结肠癌细胞系药物敏感性的表型不稳定性。
影响因子：
11.2
作者：
Ferguson,PJ;Cheng,YC
通讯作者：
Cheng,YC
顺铂加 5-FU 用于治疗结肠腺癌。
DOI：
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发表时间：
1985
期刊：
Cancer treatment reports
影响因子：
--
作者：
LoehrerSr,PJ;Einhorn,LH;Williams,SD;Hui,SL;Estes,NC;Pennington,K
通讯作者：
Pennington,K
用于估计人类研究抗癌药物临床可达到的血浆浓度的模型。
DOI：
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发表时间：
1986
期刊：
Cancer treatment reports
影响因子：
--
作者：
Scheithauer,W;Clark,GM;Salmon,SE;Dorda,W;Shoemaker,RH;VonHoff,DD
通讯作者：
VonHoff,DD