Differentiated hamster sebocytes exhibit apoptosis-resistant phenotype by the augmentation of intracellular calcium level in vitro
Differentiated hamster sebocytes exhibit apoptosis-resistant phenotype by the augmentation of intracellular calcium level in vitro
复制标题
分化的仓鼠皮脂细胞通过体外细胞内钙水平的增加表现出抗凋亡表型
DOI：
10.1111/exd.12026
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
and Ito A
中科院分区：
文献类型：
--
作者：
Kurihara H;Sato T;Akimoto N;and Ito A
皮脂腺通过将皮脂分泌到皮肤表面上而在维持皮肤屏障功能中起重要作用。在我们的研究中，我们证明，分化的仓鼠皮脂腺细胞（DHS）表现出抗凋亡和钙离子载体，A23187处理，诱导未分化的仓鼠皮脂腺细胞（unDHS）的细胞凋亡和钙离子内流的损失。DHS中细胞内Ca 2+的Fluo-3-相关信号高于unDHS，即使培养基中Ca 2+耗尽，该信号仍持续存在。此外，发现细胞内Ca 2+螯合剂BAPTA-AM可降低DHS中的Ca 2+信号，从而诱导细胞凋亡。因此，这些结果提供了新的证据，表明细胞分化依赖性增加的钙库操作的释放与DHS中的凋亡抵抗有关。此外，这些发现应加速了解生理条件下皮脂生成和/或皮脂产生和分泌的机制。
Sebaceous glands play important roles in the maintenance of the skin barrier function by secreting sebum onto the skin surface. In our study, we demonstrated that differentiated hamster sebocytes (DHS) exhibited apoptosis resistance and the loss of Ca2+influx against a calcium ionophore, A23187 treatment, which induced both apoptosis and Ca2+influx in undifferentiated hamster sebocytes (unDHS). The Fluo‐3‐related signal of intracellular Ca2+in the DHS was higher than that in unDHS and was sustained even though there was a depletion of Ca2+from the culture medium. Furthermore, the intracellular Ca2+chelator, BAPTA‐AM, was found to decrease the Ca2+signal in the DHS, which induced apoptosis. Thus, these results provide novel evidence that the cell differentiation‐dependent increase in store‐operated Ca2+release is associated with apoptosis resistance in the DHS. Moreover, these findings should accelerate the understanding of the mechanisms of sebogenesis and/or sebum production and secretion under physiological conditions.