An innovative tool for moving malaria PCR detection of parasite reservoir into the field.
An innovative tool for moving malaria PCR detection of parasite reservoir into the field.
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DOI：
10.1186/1475-2875-12-405
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发表时间：
2013-11-09
期刊：
影响因子：
3
通讯作者：
Ménard D
中科院分区：
文献类型：
--
作者：
Canier L;Khim N;Kim S;Sluydts V;Heng S;Dourng D;Eam R;Chy S;Khean C;Loch K;Ken M;Lim H;Siv S;Tho S;Masse-Navette P;Gryseels C;Uk S;Van Roey K;Grietens KP;Sokny M;Thavrin B;Chuor CM;Deubel V;Durnez L;Coosemans M;Ménard D
为了在柬埔寨等低传播地区实现消灭疟疾的目标，需要新的、廉价的、高通量的诊断工具，以确定无症状携带者中极低的寄生虫密度。这将使实地疟疾病例检测从被动转为主动。在“内部”设计的移动的实验室进行了DNA提取和实时PCR分析，从而能够在实地实施稳健、灵敏和快速的疟疾诊断战略。在MalaResT项目（NCT 01663831）背景下组织的一项调查中使用了该工具。2012年10月至11月，在拉达那基里省的移动的实验室进行了实时PCR筛查和物种鉴定分析，在不到四周的时间内筛查了大约5 000人，并在样本采集后24-48小时内治疗了寄生虫携带者。平均每天检测240份临床样本（和40份质控样本），每周6/7天。大约97.7%的结果在收集后24小时内可用。共有4.9%的人疟疾呈阳性。间日疟原虫阳性率为61.1%，恶性疟原虫阳性率为45.9%，三日疟原虫阳性率为7.0%，卵形疟原虫阳性率为2.0%。这种诊断装置的操作成功证明，分子检测和随后的治疗在实地环境中是可以实现的。这将有助于发现无症状携带者群体，并提供有用的流行病学信息。快速的结果将极大地帮助该领域的工作人员追踪和治疗无症状的寄生虫病病例。移动的实验室的概念可以推广到其他国家，用于疟疾或其他病原体的分子检测，或培养间日疟原虫，这不支持样本收集和培养之间的长时间延迟。
To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field. DNA extraction and real-time PCR assays were implemented in an “in-house” designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831). The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Rattanakiri Province, to screen approximately 5,000 individuals in less than four weeks and treat parasite carriers within 24–48 hours after sample collection. An average of 240 clinical samples (and 40 quality control samples) was tested every day, six/seven days per week. Some 97.7% of the results were available <24 hours after the collection. A total of 4.9% were positive for malaria. Plasmodium vivax was present in 61.1% of the positive samples, Plasmodium falciparum in 45.9%, Plasmodium malariae in 7.0% and Plasmodium ovale in 2.0%. The operational success of this diagnostic set-up proved that molecular testing and subsequent treatment is logistically achievable in field settings. This will allow the detection of clusters of asymptomatic carriers and to provide useful epidemiological information. Fast results will be of great help for staff in the field to track and treat asymptomatic parasitaemic cases. The concept of the mobile laboratory could be extended to other countries for the molecular detection of malaria or other pathogens, or to culture vivax parasites, which does not support long-time delay between sample collection and culture.
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影响因子：
3
作者：
Steenkeste N;Incardona S;Chy S;Duval L;Ekala MT;Lim P;Hewitt S;Sochantha T;Socheat D;Rogier C;Mercereau-Puijalon O;Fandeur T;Ariey F
通讯作者：
Ariey F
一种针对线粒体基因组的新型单扩增 PCR 在诊断挪威卑尔根回国旅行者的疟疾方面具有高度敏感性和特异性。
影响因子：
3
作者：
Haanshuus CG;Mohn SC;Mørch K;Langeland N;Blomberg B;Hanevik K
通讯作者：
Hanevik K
影响因子：
2.7
作者：
Cui, Liwang;Yan, Guiyun;Sattabongkot, Jetsumon;Cao, Yaming;Chen, Bin;Chen, Xiaoguang;Fan, Qi;Fang, Qiang;Jongwutiwes, Somchai;Parker, Daniel;Sirichaisinthop, Jeeraphat;Kyaw, Myat Phone;Su, Xin-zhuan;Yang, Henglin;Yang, Zhaoqing;Wang, Baomin;Xu, Jianwei;Zheng, Bin;Zhong, Daibin;Zhou, Guofa
通讯作者：
Zhou, Guofa
DOI：
10.1385/1-59259-271-6:189
发表时间：
2002-01-01
期刊：
Methods in molecular medicine
影响因子：
--
作者：
Snounou, Georges;Singh, Balbir
通讯作者：
Singh, Balbir
影响因子：
56.3
作者：
Amaratunga, Chanaki;Sreng, Sokunthea;Suon, Seila;Phelps, Erika S.;Stepniewska, Kasia;Lim, Pharath;Zhou, Chongjun;Mao, Sivanna;Anderson, Jennifer M.;Lindegardh, Niklas;Jiang, Hongying;Song, Jianping;Su, Xin-zhuan;White, Nicholas J.;Dondorp, Arjen M.;Anderson, Tim J. C.;Fay, Michael P.;Mu, Jianbing;Duong, Socheat;Fairhurst, Rick M.
通讯作者：
Fairhurst, Rick M.