Effect of quercetin and its conjugated metabolite on the hydrogen peroxide-induced intracellular production of reactive oxygen species in mouse fibroblasts
Effect of quercetin and its conjugated metabolite on the hydrogen peroxide-induced intracellular production of reactive oxygen species in mouse fibroblasts
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DOI：
10.1271/bbb.66.1015
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发表时间：
2002-05-01
影响因子：
1.6
通讯作者：
Terao, J
中科院分区：
文献类型：
--
作者：
Shirai, M;Yamanishi, R;Terao, J
为了阐明槲皮素代谢产物在细胞氧化应激中的抗氧化作用，我们测定了槲皮素苷元和槲皮素3-O-β-D-葡萄糖醛酸苷（Q3 GA）（摄入富含槲皮素的食物后血液中的槲皮素代谢产物之一）对小鼠成纤维细胞3 T3培养细胞中过氧化氢（H-2 O-2）诱导的细胞内活性氧产生的抑制作用。当细胞在槲皮素或Q3 GA存在下暴露于H2 O2时，发现Q3 GA的效果不如槲皮素。在接触前用槲皮素或Q3 GA预处理的情况下，Q3 GA而不是槲皮素苷元产生抑制作用，尽管其细胞摄取是不可能的。槲皮素苷元似乎未能在其抗氧化作用，由于代谢转化为异鼠李素共轭物，与大量的氧化降解导致的预处理。这表明槲皮素代谢产物参与了H_2O_2对细胞内氧化应激的保护作用。
To clarify the antioxidative role of quercetin metabolites in cellular oxidative stress, we measured the inhibitory effects of the quercetin aglycon and quercetin 3-O-beta-D-glueuronide (Q3GA), which is one of the quercetin metabolites in the blood after an intake of quercetin-rich food, on the production of hydrogen peroxide (H-2 O-2)-induced intracellular reactive oxygen species in mouse fibroblast 3T3 cultured cells. When the cells were exposed to H2O2 in the presence of quercetin or Q3GA, Q3GA was found to be less effective than quercetin. In the case of a pretreatment with quercetin or Q3GA before the exposure, Q3GA, but not the quercetin aglycon, exerted an inhibitory effect, although its cellular uptake was unlikely. The quercetin aglycon appeared to fail in its antioxidative effect due to metabolic conversion into isorhamnetin conjugates, with substantial oxidative degradation resulting from the pretreatment. It is, therefore, suggested that quercetin metabolites take part in the protection of intracellular oxidative stress induced by the extraneous attack Of H2O2.