Heat shock proteins affect RNA processing during the heat shock response of Saccharomyces cerevisiae.
Heat shock proteins affect RNA processing during the heat shock response of Saccharomyces cerevisiae.
复制标题
热休克蛋白在酿酒酵母的热休克反应过程中影响 RNA 加工。
DOI：
10.1128/mcb.11.2.1062-1068.1991
复制
发表时间：
1991
影响因子：
5.3
通讯作者：
Lindquist,S
中科院分区：
文献类型：
--
作者：
Yost,HJ;Lindquist,S
在酿酒酵母中，mRNA前体的剪接被严重的热休克破坏。在严重热休克之前进行温和的热处理可以保护剪接不被破坏，正如先前对黑腹果蝇的报道。与D.在预处理过程中黑腹菌蛋白质的合成不需要保护酵母细胞中的剪接。然而，一旦剪接被突然的严重热休克破坏，则需要蛋白质合成来快速恢复剪接。两类酵母热休克蛋白基因的突变影响热休克反应期间RNA剪接的模式。首先，某些hsp70突变体，在常温下过量产生其他热休克蛋白，在高温下显示剪接的组成性保护，不需要预处理。第二，在hsp104突变体中，与野生型细胞相比，严重热休克后RNA剪接的恢复延迟。这些结果表明，热休克蛋白的保护功能比以前怀疑的更大程度的专业化。一些蛋白质（例如，HSP 70和HSP 82基因家族的成员）有助于在较高温度下维持正常的细胞过程。hspl04的特殊功能（至少在剪接中）是在该过程被破坏后促进其恢复。
In the yeast Saccharomyces cerevisiae the splicing of mRNA precursors is disrupted by a severe heat shock. Mild heat treatments prior to severe heat shock protect splicing from disruption, as was previously reported forDrosophila melanogaster. In contrast to D. melanogaster protein synthesis during the pretreatment is not required to protect splicing in yeast cells. However, protein synthesis is required for the rapid recovery of splicing once it has been disrupted by a sudden severe heat shock. Mutations in two classes of yeast hsp genes affect the pattern of RNA splicing during the heat shock response. First, certainhsp70mutants, which overproduce other heat shock proteins at normal temperatures, show constitutive protection of splicing at high temperatures and do not require pretreatment. Second, inhsp104mutants, the recovery of RNA splicing after a severe heat shock is delayed compared with wild-type cells. These results indicate a greater degree of specialization in the protective functions of hsps than has previously been suspected. Some of the proteins (e.g., members of the hsp70 and hsp82 gene families) help to maintain normal cellular processes at higher temperatures. The particular function of hspl04, at least in splicing, is to facilitate recovery of the process once it has been disrupted.