Towards more precise definition of conditions for satisfactory deep etching
Towards more precise definition of conditions for satisfactory deep etching
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更精确地定义令人满意的深蚀刻条件
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
G. Haggis
中科院分区：
文献类型：
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作者：
G. Haggis
进行了实验，以确定丙烷喷射冷冻是否可以作为深蚀刻工作中的冲击冷冻令人满意。所使用的材料是Heuser及其同事之前研究过的肠道刷状缘，其中肌动蛋白根丝的5-6纳米装饰以及连接这些根丝的细丝网络提供了良好的标准来判断制剂的质量，关于冰晶生长和表面污染。丙烷喷射冷冻确实令人满意，只要满足适当的条件（即薄样品，表面附近的断裂）。获得了不同的结果，直到意识到使用配有旋转样品台的巴尔泽冷冻蚀刻装置，在记录热电偶显示已获得给定温度的时间与样品块实际达到该温度的时间之间有10-15分钟的延迟（当样品温度重置时）。必须对这种滞后作出适当的容差，以获得令人满意的深蚀刻复制品。
Experiments were carried out to determine whether propane‐jet freezing could be as satisfactory as impact freezing in deep etch work. The material used was the intestinal brush border, previously studied by Heuser and coworkers where the 5–6 nm decoration of actin rootlet filaments, and the fine network of filaments linking these rootlets, provide good criteria by which to judge the quality of the preparation, as regards ice crystal growth and surface contamination. Propane jet freezing was indeed found satisfactory provided appropriate conditions were met (viz thin specimen, fracture near the surface). Variable results were obtained until it was realized that with a Balzers freeze‐etch unit fitted with a rotating specimen table there is a 10–15 min delay (when specimen temperature is reset) between the time the recording thermocouple shows a given temperature to have been obtained and the time the specimen block actually reaches this temperature. Appropriate allowance must be made for this lag to achieve satisfactory deep etch replicas.