Inhibition of rust-induced hypersensitive response in flax cells by the microtubule inhibitor oryzalin
Inhibition of rust-induced hypersensitive response in flax cells by the microtubule inhibitor oryzalin
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DOI：
10.1071/pp97060
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发表时间：
1997-01-01
期刊：
影响因子：
--
通讯作者：
Hardham, AR
中科院分区：
文献类型：
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作者：
Kobayashi, I;Kobayashi, Y;Hardham, AR
亚麻锈病菌与亚麻互作的研究。是由基因对基因的关系决定的，这种关系决定了病原体的毒力或无毒力以及宿主的抗性或易感性。本研究表明，不同的敏感性M。亚麻和亚麻与微管解聚药物安磺灵的结合，使得可以在亚麻细胞中而不是在真菌细胞中获得微管解聚。通常，在不相容的相互作用中，接种后约24小时的快速过敏反应抑制真菌的发育和入侵。然而，在一个不相容的相互作用中，在安磺灵的存在下，过敏性细胞死亡的发生被推迟，其频率降低。这使得一个正常无毒的M. Lini在活的寄主叶肉细胞中形成吸器的速率和效率与在接种后的前36小时内在相容性相互作用中由毒力小种实现的速率和效率相似。此后，过敏性细胞死亡的发生率增加，病原体的进一步发展被阻止。结果表明，微管在亚麻与亚麻锈菌的小种-品种特异性互作中起着快速、高效的过敏反应。
Interactions between the flax rust fungus Melampsora lini and flax Linum usitatissimum L. are governed by a gene-for-gene relationship which determines pathogen virulence or avirulence and host resistance or susceptibility. The present study demonstrates differential sensitivity of M. lini and flax to the microtubule depolymerising drug, oryzalin, such that microtubule depolymerisation in flax cells but not in fungal cells could be obtained. Normally, in an incompatible interaction, a rapid hypersensitive response about 24 h after inoculation inhibits fungal development and invasion. However, in an incompatible interaction in the presence of oryzalin, the occurrence of hypersensitive cell death was delayed and its frequency reduced. This allowed a normally avirulent race of M. lini to form haustoria in living host mesophyll cells at a rate and efficiency similar to that achieved by a virulent race in a compatible interaction during the first 36 h after inoculation. After that time, the incidence of hypersensitive cell death increased and further development of the pathogen was arrested. The results indicate that microtubules play a role in effecting rapid and efficient hypersensitive response in the race-cultivar specific interaction between flax and the flax rust fungus.