Plasmid-mediated and inducible cephalosporinase DHA-2 from Klebsiella pneumoniae
Plasmid-mediated and inducible cephalosporinase DHA-2 from Klebsiella pneumoniae
复制标题
DOI：
10.1093/jac/47.2.207
复制
发表时间：
2001-02-01
影响因子：
5.2
通讯作者：
Nordmann, P
中科院分区：
文献类型：
--
作者：
Fortineau, N;Poirel, L;Nordmann, P
1992年，从法国巴黎住院的一名儿童中培养出对头孢西丁和氧亚氨基头孢菌素耐药的肺炎克雷伯菌菌株。该分离株携带β-内酰胺酶基因，位于约200 kb的非自身转移质粒上。所鉴定的β-内酰胺酶DHA-2与摩氏摩根菌的AmpC酶具有99%的氨基酸同一性。DHA-2是先前在沙门氏菌分离株中鉴定的DHA-I的点突变衍生物。DHA-2的表达是可诱导的，由于ampR调控基因。这是首次从克雷伯氏菌中发现一种可诱导的质粒定位的头孢菌素酶。肺炎。
A Klebsiella pneumoniae strain resistant to cefoxitin and oxyimino-cephalosporins was cultured from a child hospitalized in Paris, France, in 1992. This isolate harboured a beta -lactamase gene located on an approximately 200 kb non-self-transferable plasmid. The beta -lactamase identified, DHA-2, shared 99% amino acid identity with the AmpC enzyme of Morganella morganii. DHA-2 was a point-mutant derivative of DHA-I identified previously in a Salmonella enteritidis isolate. DHA-2 expression was inducible due to an ampR regulatory gene. This is the first report of an inducible and plasmid-located cephalosporinase from K. pneumoniae.